Identification and validation of reference genes for qPCR of Pseudomonas aeruginosa L10 under varying n-hexadecane concentrations

Identification and validation of reference genes for qPCR of Pseudomonas aeruginosa L10 under varying n-hexadecane concentrations

Abstract Pseudomonas aeruginosa can efficiently degrades petroleum hydrocarbons, but its degradation rate is affected by hydrocarbon pollutant concentrations, which must be neither too high nor too low. To quantitatively analyze P. aeruginosa’s gene expression and assess factors affecting it, refere...

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Bibliographic Details
Main Authors: Huan Wang, Xiaobing Wang, Juntao Chen, Yumiao Zhang, Junhua Liu, Fangliang Xia, Jian Li, Tao Wu, Longxiang Liu
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Microbiology
Subjects:
Bioremediation
N-hexadecane
Pseudomonas aeruginosa
Quantitative real-time PCR
Reference gene
Online Access:https://doi.org/10.1186/s12866-025-04112-2
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https://doi.org/10.1186/s12866-025-04112-2

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