Multiplexed CRISPR Assay for Amplification-Free Detection of miRNAs

Multiplexed CRISPR Assay for Amplification-Free Detection of miRNAs

CRISPR-Cas proteins from bacteria are powerful tools for gene editing and molecular diagnostics. Expanding capacity of CRISPR to low cost, multiplexed assays of biomarkers is a key to future disease diagnostics, since multiple biomarker detection is essential for reliable diagnostics. Herein we desc...

Full description

Saved in:

Bibliographic Details
Main Authors:	P. I. Thilini De Silva, Keshani Hiniduma, Rachelle Canete, Ketki S. Bhalerao, Sherif M. Shawky, Hansana Gunathilaka, Jessica L. Rouge, Islam M. Mosa, David C. Steffens, Kevin Manning, Breno S. Diniz, James F. Rusling
Format:	Article
Language:	English
Published:	MDPI AG 2025-05-01
Series:	Biosensors
Subjects:	CRISPR Alzheimer biomarkers miRNAs multiplexed assay fluorescence
Online Access:	https://www.mdpi.com/2079-6374/15/6/346
Tags:	Add Tag No Tags, Be the first to tag this record!

Similar Items

Multiplex detection of respiratory RNA viruses without amplification based on CRISPR-Cas13a immunochromatographic test strips
by: Tao Wang, et al.
Published: (2025-06-01)

Applications of multiplexed CRISPR–Cas for genome engineering
by: Himchan Cheng, et al.
Published: (2025-07-01)

Multiplexing using isothermal Recombinase Polymerase Amplification (RPA).
Published: (2017-10-01)

Heritable multiplex genetic engineering in rats using CRISPR/Cas9.
by: Yuanwu Ma, et al.
Published: (2014-01-01)

Amplification technology for spatial division multiplexing signals transmitted using multicore fibres
by: Hitoshi Takeshita
Published: (2024-12-01)

Multiplex Ligation-Dependent Probe Amplification to Detect HER2 Amplification in Breast Cancer: New Insights in Optimal Cut-Off Value
by: Cathy B. Moelans, et al.
Published: (2010-01-01)

DNA Molecular Computing with Weighted Signal Amplification for Cancer miRNA Biomarker Diagnostics
by: Hongyang Zhao, et al.
Published: (2025-06-01)

A Novel miRNA Detection Method Using Loop-Mediated Isothermal Amplification
by: Saiwei Wu, et al.
Published: (2023-01-01)

Rapid and Visual Detection of Mycobacterium tuberculosis Complex Based on Multiplex Recombinase Polymerase Amplification
by: Xiaoyan Tan, et al.
Published: (2025-06-01)

ESR1 Amplification is Rare in Breast Cancer and is Associated with High Grade and High Proliferation: A Multiplex Ligation-Dependent Probe Amplification Study
by: Cathy B. Moelans, et al.
Published: (2010-01-01)

Ultrasensitive and Multiplexed Target Detection Strategy Based on Photocleavable Mass Tags and Mass Signal Amplification
by: Seokhwan Ji, et al.
Published: (2025-07-01)

Multiplex Ligation Probe Amplification and Sanger Sequencing: Light and Shade in the Diagnosis of Lysosomal Storage Disorders
by: Martina Vinci, et al.
Published: (2025-04-01)

Detection of Neisseria gonorrhoeae and Chlamydia trachomatis infections in pregnant women by multiplex recombinase polymerase amplification.
by: Jingjing Zhai, et al.
Published: (2021-01-01)

Programmable Multiplexed Nucleic Acid Detection by Harnessing Specificity Defect of CRISPR‐Cas12a
by: Xin Guan, et al.
Published: (2025-01-01)

Establishment of the CRISPR-Cpf1 gene editing system in Bacillus licheniformis and multiplexed gene knockout
by: Suxin Liu, et al.
Published: (2025-03-01)

A multifunctional switch for label-free CRISPR/Cas12a sensor with self-driven amplification
by: Po Li, et al.
Published: (2025-12-01)

Rapid detection of Mycoplasma hyopneumoniae by recombinase-aided amplification combined with the CRISPR/Cas12a system
by: Kaili Li, et al.
Published: (2024-12-01)

Efficient, cell-type-specific production of flavonols by multiplexed CRISPR activation of a suite of metabolic enzymes
by: Anaxi Houbaert, et al.
Published: (2025-07-01)

Detection of HER2 Amplification in Breast Carcinomas: Comparison of Multiplex Ligation-Dependent Probe Amplification (MLPA) and Fluorescence In Situ Hybridization (FISH) combined with Automated Spot Counting
by: Elna Moerland, et al.
Published: (2006-01-01)

One-Pot Detection of miRNA by Dual Rolling Circle Amplification at Ambient Temperature with High Specificity and Sensitivity
by: Wenhua Sun, et al.
Published: (2025-05-01)

Framework nucleic acid-based linear amplification platform for sensitive detection of bladder cancer-related miRNAs
by: MAO Chenzhou, et al.
Published: (2025-03-01)

Genogroup-Specific Multiplex Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Point-of-Care Detection of Norovirus
by: Wahedul Karim Ansari, et al.
Published: (2025-07-01)

A combination of recombinase polymerase amplification with CRISPR technology rapidly detects goose parvovirus with high accuracy and sensitivity
by: Xiuqin Chen, et al.
Published: (2025-06-01)

Selective targeting of genome amplifications and repeat elements by CRISPR-Cas9 nickases to promote cancer cell death
by: Matthew B. Hanlon, et al.
Published: (2025-06-01)

Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor
by: Mingyuan Sun, et al.
Published: (2025-04-01)

Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells
by: Viktor Glaser, et al.
Published: (2023-04-01)

CRISPR/Cas9 multiplex genome editing to enhance grain lysine concentration in a US rice cultivar
by: Khushboo Rastogi, et al.
Published: (2025-06-01)

Deep Learning‐Enhanced Hand‐Driven Microfluidic Chip for Multiplexed Nucleic Acid Detection Based on RPA/CRISPR
by: Tao Xu, et al.
Published: (2025-06-01)

转基因作物多重重组酶聚合酶扩增结合CRISPR/Cas12a现场可视化检测方法的建立(英文)(Establishment of a field visualization detection method for multiplex recombinase polymerase amplification combined with CRISPR/Cas12a in genetically modified crops)
by: 颜晶莹(YAN Jingying), et al.
Published: (2025-06-01)

Autonomous microfluidic influenza A/B subtyping system using on-chip multiplex isothermal amplification for field-deployable surveillance
by: Si-Ming Lu, et al.
Published: (2025-08-01)

Recombinase polymerase amplification combined with CRISPR/Cas12a technology for rapid on-site detection of duck adenovirus 3
by: Qi-Zhang Liang, et al.
Published: (2025-08-01)

Rapid visual detection assay for Bactrocera dorsalis (Hendel) using recombinase polymerase amplification and CRISPR/Cas12b
by: Chenyu Lv, et al.
Published: (2025-05-01)

A CRISPR Cas12a/Cpf1 strategy to facilitate robust multiplex gene editing in Aspergillus Niger
by: Abel Peter van Esch, et al.
Published: (2025-04-01)

Targeted DNA Nanomachine Enables Specific miRNA‐Responsive Singlet Oxygen Amplification for Precise Cutaneous Squamous Cancer Therapy
by: Hanane Aliouat, et al.
Published: (2025-05-01)

Optimization of a thermochemical antibody stripping method for enhanced tyramide signal amplification-based opal multiplex immunohistochemistry in fragile tissues
by: Xiaotong Yu, et al.
Published: (2025-05-01)

Development of multiplexed reverse-transcription loop-mediated isothermal amplification for detection of SARS-CoV-2 and influenza viral RNA
by: Yinhua Zhang, et al.
Published: (2021-03-01)

Amplification-Free Electrochemiluminescent Biosensor for Ultrasensitive Detection of Fusobacterium nucleatum Using Tetrahedral DNA-Based CRISPR/Cas12a
by: Xindan Zhang, et al.
Published: (2025-01-01)

Establishment and optimization of a system for the detection of Candida albicans based on enzymatic recombinase amplification and CRISPR/Cas12a system
by: Xiaotong Zeng, et al.
Published: (2025-05-01)

Ultrasensitive detection of clinical pathogens through a target-amplification-free collateral-cleavage-enhancing CRISPR-CasΦ tool
by: Huiyou Chen, et al.
Published: (2025-04-01)

Unveiling Cas12j Trans‐Cleavage Activity for CRISPR Diagnostics: Application to miRNA Detection in Lung Cancer Diagnosis
by: Ju‐Eun Kang, et al.
Published: (2024-11-01)