Skeletal Site-Specific Lipid Profile and Hematopoietic Progenitors of Bone Marrow Adipose Tissue in Patients Undergoing Primary Hip Arthroplasty
Background/Objectives: Bone marrow adipose tissue (BMAT) has been described as an important biomechanic and lipotoxic factor with negative impacts on skeletal and hematopoietic system regeneration. BMAT undergoes metabolic and cellular adaptations with age and disease, being a source of potential bi...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/15/1/16 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588007828357120 |
|---|---|
| author | Drenka Trivanović Marko Vujačić Aleksandra Arsić Tamara Kukolj Milica Rajković Nikola Bogosavljević Zoran Baščarević Mirjana Maljković Ružičić Jovana Kovačević Aleksandra Jauković |
| author_facet | Drenka Trivanović Marko Vujačić Aleksandra Arsić Tamara Kukolj Milica Rajković Nikola Bogosavljević Zoran Baščarević Mirjana Maljković Ružičić Jovana Kovačević Aleksandra Jauković |
| author_sort | Drenka Trivanović |
| collection | DOAJ |
| description | Background/Objectives: Bone marrow adipose tissue (BMAT) has been described as an important biomechanic and lipotoxic factor with negative impacts on skeletal and hematopoietic system regeneration. BMAT undergoes metabolic and cellular adaptations with age and disease, being a source of potential biomarkers. However, there is no evidence on the lipid profile and cellularity at different skeletal locations in osteoarthritis patients undergoing primary hip arthroplasty. Methods: Acetabular and femoral bone marrow (BM) and gluteofemoral subcutaneous adipose tissue (gfSAT) were obtained from matched patients undergoing hip replacement surgery. BM, BMAT, and gfSAT were explored at the levels of total lipids, fatty acids, and cells by using thin-layerand gas chromatography, ex vivo cellular assays, and flow cytometry. Results: BMAT content was significantly higher in femoral than in acetabular BM. Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and gfSAT. Frequencies of saturated palmitic, myristic, and stearic acids were higher in femoral than in acetabular BMAT and gfSAT. The content of CD45<sup>+</sup>CD34<sup>+</sup> cells within femoral BMAT was higher than in acetabular BMAT or gfSAT. This was associated with a higher incidence of total clonogenic hematopoietic progenitors and late erythroid colonies CFU-E in femoral BMAT when compared to acetabular BMAT, similar to their BM counterparts. Conclusions: Collectively, our results indicate that the lipid profiles of hip bone and femoral BMAT impose significantly different microenvironments and distributions of cells with hematopoietic potential. These findings might bring forth new inputs for defining BMAT biology and setting novel directions in OA disease investigations. |
| format | Article |
| id | doaj-art-9b4bc7ad23a742ada5000cd3b2207a7d |
| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj-art-9b4bc7ad23a742ada5000cd3b2207a7d2025-01-24T13:41:10ZengMDPI AGMetabolites2218-19892025-01-011511610.3390/metabo15010016Skeletal Site-Specific Lipid Profile and Hematopoietic Progenitors of Bone Marrow Adipose Tissue in Patients Undergoing Primary Hip ArthroplastyDrenka Trivanović0Marko Vujačić1Aleksandra Arsić2Tamara Kukolj3Milica Rajković4Nikola Bogosavljević5Zoran Baščarević6Mirjana Maljković Ružičić7Jovana Kovačević8Aleksandra Jauković9Group for Hematology and Stem Cells, Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaInstitute for Orthopedy Banjica, 11000 Belgrade, SerbiaCentre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaGroup for Hematology and Stem Cells, Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaGroup for Hematology and Stem Cells, Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaInstitute for Orthopedy Banjica, 11000 Belgrade, SerbiaInstitute for Orthopedy Banjica, 11000 Belgrade, SerbiaFaculty of Mathematics, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Mathematics, University of Belgrade, 11000 Belgrade, SerbiaGroup for Hematology and Stem Cells, Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaBackground/Objectives: Bone marrow adipose tissue (BMAT) has been described as an important biomechanic and lipotoxic factor with negative impacts on skeletal and hematopoietic system regeneration. BMAT undergoes metabolic and cellular adaptations with age and disease, being a source of potential biomarkers. However, there is no evidence on the lipid profile and cellularity at different skeletal locations in osteoarthritis patients undergoing primary hip arthroplasty. Methods: Acetabular and femoral bone marrow (BM) and gluteofemoral subcutaneous adipose tissue (gfSAT) were obtained from matched patients undergoing hip replacement surgery. BM, BMAT, and gfSAT were explored at the levels of total lipids, fatty acids, and cells by using thin-layerand gas chromatography, ex vivo cellular assays, and flow cytometry. Results: BMAT content was significantly higher in femoral than in acetabular BM. Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and gfSAT. Frequencies of saturated palmitic, myristic, and stearic acids were higher in femoral than in acetabular BMAT and gfSAT. The content of CD45<sup>+</sup>CD34<sup>+</sup> cells within femoral BMAT was higher than in acetabular BMAT or gfSAT. This was associated with a higher incidence of total clonogenic hematopoietic progenitors and late erythroid colonies CFU-E in femoral BMAT when compared to acetabular BMAT, similar to their BM counterparts. Conclusions: Collectively, our results indicate that the lipid profiles of hip bone and femoral BMAT impose significantly different microenvironments and distributions of cells with hematopoietic potential. These findings might bring forth new inputs for defining BMAT biology and setting novel directions in OA disease investigations.https://www.mdpi.com/2218-1989/15/1/16bone marrow adipose tissueosteoarthritisfatty acidstem cellshematopoietic progenitors |
| spellingShingle | Drenka Trivanović Marko Vujačić Aleksandra Arsić Tamara Kukolj Milica Rajković Nikola Bogosavljević Zoran Baščarević Mirjana Maljković Ružičić Jovana Kovačević Aleksandra Jauković Skeletal Site-Specific Lipid Profile and Hematopoietic Progenitors of Bone Marrow Adipose Tissue in Patients Undergoing Primary Hip Arthroplasty Metabolites bone marrow adipose tissue osteoarthritis fatty acid stem cells hematopoietic progenitors |
| title | Skeletal Site-Specific Lipid Profile and Hematopoietic Progenitors of Bone Marrow Adipose Tissue in Patients Undergoing Primary Hip Arthroplasty |
| title_full | Skeletal Site-Specific Lipid Profile and Hematopoietic Progenitors of Bone Marrow Adipose Tissue in Patients Undergoing Primary Hip Arthroplasty |
| title_fullStr | Skeletal Site-Specific Lipid Profile and Hematopoietic Progenitors of Bone Marrow Adipose Tissue in Patients Undergoing Primary Hip Arthroplasty |
| title_full_unstemmed | Skeletal Site-Specific Lipid Profile and Hematopoietic Progenitors of Bone Marrow Adipose Tissue in Patients Undergoing Primary Hip Arthroplasty |
| title_short | Skeletal Site-Specific Lipid Profile and Hematopoietic Progenitors of Bone Marrow Adipose Tissue in Patients Undergoing Primary Hip Arthroplasty |
| title_sort | skeletal site specific lipid profile and hematopoietic progenitors of bone marrow adipose tissue in patients undergoing primary hip arthroplasty |
| topic | bone marrow adipose tissue osteoarthritis fatty acid stem cells hematopoietic progenitors |
| url | https://www.mdpi.com/2218-1989/15/1/16 |
| work_keys_str_mv | AT drenkatrivanovic skeletalsitespecificlipidprofileandhematopoieticprogenitorsofbonemarrowadiposetissueinpatientsundergoingprimaryhiparthroplasty AT markovujacic skeletalsitespecificlipidprofileandhematopoieticprogenitorsofbonemarrowadiposetissueinpatientsundergoingprimaryhiparthroplasty AT aleksandraarsic skeletalsitespecificlipidprofileandhematopoieticprogenitorsofbonemarrowadiposetissueinpatientsundergoingprimaryhiparthroplasty AT tamarakukolj skeletalsitespecificlipidprofileandhematopoieticprogenitorsofbonemarrowadiposetissueinpatientsundergoingprimaryhiparthroplasty AT milicarajkovic skeletalsitespecificlipidprofileandhematopoieticprogenitorsofbonemarrowadiposetissueinpatientsundergoingprimaryhiparthroplasty AT nikolabogosavljevic skeletalsitespecificlipidprofileandhematopoieticprogenitorsofbonemarrowadiposetissueinpatientsundergoingprimaryhiparthroplasty AT zoranbascarevic skeletalsitespecificlipidprofileandhematopoieticprogenitorsofbonemarrowadiposetissueinpatientsundergoingprimaryhiparthroplasty AT mirjanamaljkovicruzicic skeletalsitespecificlipidprofileandhematopoieticprogenitorsofbonemarrowadiposetissueinpatientsundergoingprimaryhiparthroplasty AT jovanakovacevic skeletalsitespecificlipidprofileandhematopoieticprogenitorsofbonemarrowadiposetissueinpatientsundergoingprimaryhiparthroplasty AT aleksandrajaukovic skeletalsitespecificlipidprofileandhematopoieticprogenitorsofbonemarrowadiposetissueinpatientsundergoingprimaryhiparthroplasty |