Evaluation of normalisation strategies for qPCR data obtained from canine gastrointestinal tissues with different pathologies

Evaluation of normalisation strategies for qPCR data obtained from canine gastrointestinal tissues with different pathologies

Abstract Quantitative real-time PCR (qPCR) is widely used to quantify gene expression at the mRNA level and confirm RNAseq results. Normalisation is a critical process used to minimise technical variability introduced during sample processing. To achieve this, reference genes (RGs), known for their...

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Bibliographic Details
Main Authors: Maria G. Luigi-Sierra, Janne G. Lyngby, Ann-Sofie Ingerslev, Jennifer Mari Jacobsen, Lise N. Nielsen, Susanna Cirera
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Subjects:
Dog
Gastrointestinal tract
Chronic inflammatory enteropathy
Gastrointestinal cancer
qPCR
Normalisation
Online Access:https://doi.org/10.1038/s41598-025-06657-8
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https://doi.org/10.1038/s41598-025-06657-8

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