Encapsulation of Japanese grape (Hovenia dulcis) pseudofruits by freeze drying: characterization and antioxidant potential

The pseudofruit of Hovenia dulcis is recognized as a source of bioactive compounds; however, like most fruits, it is highly susceptible to deterioration. Encapsulation techniques are used to protect and stabilize compounds while also minimizing changes in the properties of the supplemented product....

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Main Authors: Elisandra Rigo, Dalana Cecília Hanauer, Leticia Knakiewicz, Georgia Ane Raquel Sehn, Darlene Cavalheiro
Format: Article
Language:English
Published: Instituto Federal de Educação, Ciência e Tecnologia da Paraíba 2025-01-01
Series:Revista Principia
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Online Access:https://periodicos.ifpb.edu.br/index.php/principia/article/view/8259
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author Elisandra Rigo
Dalana Cecília Hanauer
Leticia Knakiewicz
Georgia Ane Raquel Sehn
Darlene Cavalheiro
author_facet Elisandra Rigo
Dalana Cecília Hanauer
Leticia Knakiewicz
Georgia Ane Raquel Sehn
Darlene Cavalheiro
author_sort Elisandra Rigo
collection DOAJ
description The pseudofruit of Hovenia dulcis is recognized as a source of bioactive compounds; however, like most fruits, it is highly susceptible to deterioration. Encapsulation techniques are used to protect and stabilize compounds while also minimizing changes in the properties of the supplemented product. This study analyzed freeze-dried pulp of H. dulcis pseudofruit (HD) and pseudofruit pulp microencapsulated by freeze-drying using whey protein concentrate and gum arabic as coating materials (En-HD). The samples (HD and En-HD) were characterized for their physicochemical properties, total phenolic compounds (TPC), antioxidant activity (ABTS and DPPH), α-amylase inhibition, particle size distribution, and scanning electron microscopy. Microencapsulation retained 95.8% of TPC after 75 days of storage at –80 ºC. En-HD showed higher antioxidant activity by the ABTS assay and greater inhibition of the α-amylase enzyme, demonstrating the protective effect of microencapsulation. This technique, with further testing, particularly in vivo, could be promising for managing blood sugar levels. En-HD exhibited a smaller particle size and 76% greater solubility than HD, likely due to the coating materials used in the microencapsulation process and the freeze-drying of the encapsulated sample made with previously freeze-dried HD. En-HD shows potential as a functional additive for use in food industry.
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spelling doaj-art-9b1f787858264d76b5abc4b28b160f4a2025-08-20T02:51:18ZengInstituto Federal de Educação, Ciência e Tecnologia da ParaíbaRevista Principia1517-03062447-91872025-01-016210.18265/2447-9187a2024id82596479Encapsulation of Japanese grape (Hovenia dulcis) pseudofruits by freeze drying: characterization and antioxidant potentialElisandra Rigo0https://orcid.org/0000-0002-5405-5168Dalana Cecília Hanauer1https://orcid.org/0000-0001-6333-2983Leticia Knakiewicz2https://orcid.org/0000-0003-1325-3819Georgia Ane Raquel Sehn3https://orcid.org/0000-0002-3780-3670Darlene Cavalheiro4https://orcid.org/0000-0003-2556-0277Universidade do Estado de Santa Catarina (UDESC), Pinhalzinho, Santa Catarina, Universidade do Estado de Santa Catarina (UDESC), Pinhalzinho, Santa Catarina, Universidade do Estado de Santa Catarina (UDESC), Pinhalzinho, Santa Catarina, Universidade do Estado de Santa Catarina (UDESC), Pinhalzinho, Santa Catarina, Universidade do Estado de Santa Catarina (UDESC), Pinhalzinho, Santa Catarina, The pseudofruit of Hovenia dulcis is recognized as a source of bioactive compounds; however, like most fruits, it is highly susceptible to deterioration. Encapsulation techniques are used to protect and stabilize compounds while also minimizing changes in the properties of the supplemented product. This study analyzed freeze-dried pulp of H. dulcis pseudofruit (HD) and pseudofruit pulp microencapsulated by freeze-drying using whey protein concentrate and gum arabic as coating materials (En-HD). The samples (HD and En-HD) were characterized for their physicochemical properties, total phenolic compounds (TPC), antioxidant activity (ABTS and DPPH), α-amylase inhibition, particle size distribution, and scanning electron microscopy. Microencapsulation retained 95.8% of TPC after 75 days of storage at –80 ºC. En-HD showed higher antioxidant activity by the ABTS assay and greater inhibition of the α-amylase enzyme, demonstrating the protective effect of microencapsulation. This technique, with further testing, particularly in vivo, could be promising for managing blood sugar levels. En-HD exhibited a smaller particle size and 76% greater solubility than HD, likely due to the coating materials used in the microencapsulation process and the freeze-drying of the encapsulated sample made with previously freeze-dried HD. En-HD shows potential as a functional additive for use in food industry.https://periodicos.ifpb.edu.br/index.php/principia/article/view/8259abtsdpphparticle sizephenolic compounds?-amylase inhibition
spellingShingle Elisandra Rigo
Dalana Cecília Hanauer
Leticia Knakiewicz
Georgia Ane Raquel Sehn
Darlene Cavalheiro
Encapsulation of Japanese grape (Hovenia dulcis) pseudofruits by freeze drying: characterization and antioxidant potential
Revista Principia
abts
dpph
particle size
phenolic compounds
?-amylase inhibition
title Encapsulation of Japanese grape (Hovenia dulcis) pseudofruits by freeze drying: characterization and antioxidant potential
title_full Encapsulation of Japanese grape (Hovenia dulcis) pseudofruits by freeze drying: characterization and antioxidant potential
title_fullStr Encapsulation of Japanese grape (Hovenia dulcis) pseudofruits by freeze drying: characterization and antioxidant potential
title_full_unstemmed Encapsulation of Japanese grape (Hovenia dulcis) pseudofruits by freeze drying: characterization and antioxidant potential
title_short Encapsulation of Japanese grape (Hovenia dulcis) pseudofruits by freeze drying: characterization and antioxidant potential
title_sort encapsulation of japanese grape hovenia dulcis pseudofruits by freeze drying characterization and antioxidant potential
topic abts
dpph
particle size
phenolic compounds
?-amylase inhibition
url https://periodicos.ifpb.edu.br/index.php/principia/article/view/8259
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