De novo basecalling of RNA modifications at single molecule and nucleotide resolution

Abstract RNA modifications influence RNA function and fate, but detecting them in individual molecules remains challenging for most modifications. Here we present a novel methodology to generate training sets and build modification-aware basecalling models. Using this approach, we develop the m 6 AB...

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Bibliographic Details
Main Authors: Sonia Cruciani, Anna Delgado-Tejedor, Leszek P. Pryszcz, Rebeca Medina, Laia Llovera, Eva Maria Novoa
Format: Article
Language:English
Published: BMC 2025-02-01
Series:Genome Biology
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Online Access:https://doi.org/10.1186/s13059-025-03498-6
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