Impact of Curcumin on Frozen Bovine Sperm Quality and In Vitro Bovine Oocyte Maturation
This study aimed to analyze the effect of curcumin on the antioxidant properties and fertility of freeze–thawed bovine spermatozoa and bovine oocytes. In this study, curcumin concentrations of 0, 5, 10, 25, and 50 µM were added bovine sperm cryopreservation solution and oocyte IVM medium to assess s...
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MDPI AG
2025-05-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/12/5/441 |
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| author | Hao Lin Zhiye Hu Yang Li Yingchun Li Wenao Ma Shoujie Zheng Jianye Zhou Zhihui Zhao Shangquan Gan Zhibao Chen Namula Zhao |
| author_facet | Hao Lin Zhiye Hu Yang Li Yingchun Li Wenao Ma Shoujie Zheng Jianye Zhou Zhihui Zhao Shangquan Gan Zhibao Chen Namula Zhao |
| author_sort | Hao Lin |
| collection | DOAJ |
| description | This study aimed to analyze the effect of curcumin on the antioxidant properties and fertility of freeze–thawed bovine spermatozoa and bovine oocytes. In this study, curcumin concentrations of 0, 5, 10, 25, and 50 µM were added bovine sperm cryopreservation solution and oocyte IVM medium to assess sperm quality, antioxidant properties, oocyte maturation, IVF rate, and embryonic development. The results demonstrated that adding curcumin to the cryopreservation solution significantly improved the viability, motility, and acrosome integrity of bull sperm after freezing and thawing (<i>p</i> < 0.05). The addition of 25 µM curcumin resulted in the best sperm quality. Analysis of antioxidant capacity showed that 25 µM curcumin significantly increased the activities of MMP and antioxidant enzymes, such as CAT, SOD, and GSH-PX, and lowered the levels of MDA and ROS (<i>p</i> < 0.05). Adding curcumin to the in vitro maturation medium notably enhanced the maturation rate and decreased DNA fragmented nuclei of bovine oocytes (<i>p</i> < 0.05), with optimal outcomes observed at 25 and 50 µM curcumin. Totals of 25 and 50 µM curcumin markedly elevated GSH and MMP (<i>p</i> < 0.05), reduced ROS and malondialdehyde concentrations (<i>p</i> < 0.05), and significantly enhanced fertilization rates and blastocyst formation (<i>p</i> < 0.05). In conclusion, incorporating curcumin into both the bovine semen cryopreservation solution and the oocyte IVM medium significantly improved the quality of frozen–thawed sperm, antioxidant activity, oocyte maturation, IVF rate, and embryonic development. |
| format | Article |
| id | doaj-art-9aeecc7c203245d1a2efc606b523cf77 |
| institution | OA Journals |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-9aeecc7c203245d1a2efc606b523cf772025-08-20T02:33:51ZengMDPI AGVeterinary Sciences2306-73812025-05-0112544110.3390/vetsci12050441Impact of Curcumin on Frozen Bovine Sperm Quality and In Vitro Bovine Oocyte MaturationHao Lin0Zhiye Hu1Yang Li2Yingchun Li3Wenao Ma4Shoujie Zheng5Jianye Zhou6Zhihui Zhao7Shangquan Gan8Zhibao Chen9Namula Zhao10College of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, ChinaAnimal Husbandry Technology Promotion Station, Zhanjiang Municipal Bureau of Agriculture and Rural Affairs, Zhanjiang 524088, ChinaAnimal Husbandry Technology Promotion Station, Zhanjiang Municipal Bureau of Agriculture and Rural Affairs, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang 524088, ChinaThis study aimed to analyze the effect of curcumin on the antioxidant properties and fertility of freeze–thawed bovine spermatozoa and bovine oocytes. In this study, curcumin concentrations of 0, 5, 10, 25, and 50 µM were added bovine sperm cryopreservation solution and oocyte IVM medium to assess sperm quality, antioxidant properties, oocyte maturation, IVF rate, and embryonic development. The results demonstrated that adding curcumin to the cryopreservation solution significantly improved the viability, motility, and acrosome integrity of bull sperm after freezing and thawing (<i>p</i> < 0.05). The addition of 25 µM curcumin resulted in the best sperm quality. Analysis of antioxidant capacity showed that 25 µM curcumin significantly increased the activities of MMP and antioxidant enzymes, such as CAT, SOD, and GSH-PX, and lowered the levels of MDA and ROS (<i>p</i> < 0.05). Adding curcumin to the in vitro maturation medium notably enhanced the maturation rate and decreased DNA fragmented nuclei of bovine oocytes (<i>p</i> < 0.05), with optimal outcomes observed at 25 and 50 µM curcumin. Totals of 25 and 50 µM curcumin markedly elevated GSH and MMP (<i>p</i> < 0.05), reduced ROS and malondialdehyde concentrations (<i>p</i> < 0.05), and significantly enhanced fertilization rates and blastocyst formation (<i>p</i> < 0.05). In conclusion, incorporating curcumin into both the bovine semen cryopreservation solution and the oocyte IVM medium significantly improved the quality of frozen–thawed sperm, antioxidant activity, oocyte maturation, IVF rate, and embryonic development.https://www.mdpi.com/2306-7381/12/5/441bovinespermfrozenIVM |
| spellingShingle | Hao Lin Zhiye Hu Yang Li Yingchun Li Wenao Ma Shoujie Zheng Jianye Zhou Zhihui Zhao Shangquan Gan Zhibao Chen Namula Zhao Impact of Curcumin on Frozen Bovine Sperm Quality and In Vitro Bovine Oocyte Maturation Veterinary Sciences bovine sperm frozen IVM |
| title | Impact of Curcumin on Frozen Bovine Sperm Quality and In Vitro Bovine Oocyte Maturation |
| title_full | Impact of Curcumin on Frozen Bovine Sperm Quality and In Vitro Bovine Oocyte Maturation |
| title_fullStr | Impact of Curcumin on Frozen Bovine Sperm Quality and In Vitro Bovine Oocyte Maturation |
| title_full_unstemmed | Impact of Curcumin on Frozen Bovine Sperm Quality and In Vitro Bovine Oocyte Maturation |
| title_short | Impact of Curcumin on Frozen Bovine Sperm Quality and In Vitro Bovine Oocyte Maturation |
| title_sort | impact of curcumin on frozen bovine sperm quality and in vitro bovine oocyte maturation |
| topic | bovine sperm frozen IVM |
| url | https://www.mdpi.com/2306-7381/12/5/441 |
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