oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21
Introduction. Dendritic cells (DCs) and oxLDL play an important role in the atherosclerotic process with DCs accumulating in the plaques during plaque progression. Our aim was to investigate the role of oxLDL in the modulation of the DC homing-receptor CCR7 and endothelial-ligand CCL21. Methods and...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/320953 |
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| author | Thomas Nickel Susanne Pfeiler Claudia Summo Reinhard Kopp Georgios Meimarakis Zeljka Sicic Marius Lambert Korbinian Lackermair Robert David Andres Beiras-Fernandez Ingo Kaczmarek Michael Weis |
| author_facet | Thomas Nickel Susanne Pfeiler Claudia Summo Reinhard Kopp Georgios Meimarakis Zeljka Sicic Marius Lambert Korbinian Lackermair Robert David Andres Beiras-Fernandez Ingo Kaczmarek Michael Weis |
| author_sort | Thomas Nickel |
| collection | DOAJ |
| description | Introduction. Dendritic cells (DCs) and oxLDL play an important role in the atherosclerotic process with DCs accumulating in the plaques during plaque progression. Our aim was to investigate the role of oxLDL in the modulation of the DC homing-receptor CCR7 and endothelial-ligand CCL21. Methods and Results. The expression of the DC homing-receptor CCR7 and its endothelial-ligand CCL21 was examined on atherosclerotic carotic plaques of 47 patients via qRT-PCR and immunofluorescence. In vitro, we studied the expression of CCR7 on DCs and CCL21 on human microvascular endothelial cells (HMECs) in response to oxLDL. CCL21- and CCR7-mRNA levels were significantly downregulated in atherosclerotic plaques versus non-atherosclerotic controls [90% for CCL21 and 81% for CCR7 (𝑃<0.01)]. In vitro, oxLDL reduced CCR7 mRNA levels on DCs by 30% and protein levels by 46%. Furthermore, mRNA expression of CCL21 was significantly reduced by 50% (𝑃<0.05) and protein expression by 24% in HMECs by oxLDL (𝑃<0.05). Conclusions. The accumulation of DCs in atherosclerotic plaques appears to be related to a downregulation of chemokines and their ligands, which are known to regulate DC migration. oxLDL induces an in vitro downregulation of CCR7 and CCL21, which may play a role in the reduction of DC migration from the plaques. |
| format | Article |
| id | doaj-art-9a6213b294fd4b368667897111153bf6 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-9a6213b294fd4b368667897111153bf62025-08-20T02:20:15ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/320953320953oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21Thomas Nickel0Susanne Pfeiler1Claudia Summo2Reinhard Kopp3Georgios Meimarakis4Zeljka Sicic5Marius Lambert6Korbinian Lackermair7Robert David8Andres Beiras-Fernandez9Ingo Kaczmarek10Michael Weis11Medizinische Klinik und Poliklinik I, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyInstitute of Clinical Chemistry, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyMedizinische Klinik und Poliklinik I, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyDepartment of Surgery, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyDepartment of Surgery, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyMedizinische Klinik und Poliklinik I, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyMedizinische Klinik und Poliklinik I, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyMedizinische Klinik und Poliklinik I, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyMedizinische Klinik und Poliklinik I, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyDepartment of Cardiothoracic Surgery, J. W. Goethe University, 61590 Frankfurt, GermanyDepartment of Cardiac Surgery, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyMedizinische Klinik und Poliklinik I, Campus Grosshadern, Ludwig-Maximilians University, 81377 Munich, GermanyIntroduction. Dendritic cells (DCs) and oxLDL play an important role in the atherosclerotic process with DCs accumulating in the plaques during plaque progression. Our aim was to investigate the role of oxLDL in the modulation of the DC homing-receptor CCR7 and endothelial-ligand CCL21. Methods and Results. The expression of the DC homing-receptor CCR7 and its endothelial-ligand CCL21 was examined on atherosclerotic carotic plaques of 47 patients via qRT-PCR and immunofluorescence. In vitro, we studied the expression of CCR7 on DCs and CCL21 on human microvascular endothelial cells (HMECs) in response to oxLDL. CCL21- and CCR7-mRNA levels were significantly downregulated in atherosclerotic plaques versus non-atherosclerotic controls [90% for CCL21 and 81% for CCR7 (𝑃<0.01)]. In vitro, oxLDL reduced CCR7 mRNA levels on DCs by 30% and protein levels by 46%. Furthermore, mRNA expression of CCL21 was significantly reduced by 50% (𝑃<0.05) and protein expression by 24% in HMECs by oxLDL (𝑃<0.05). Conclusions. The accumulation of DCs in atherosclerotic plaques appears to be related to a downregulation of chemokines and their ligands, which are known to regulate DC migration. oxLDL induces an in vitro downregulation of CCR7 and CCL21, which may play a role in the reduction of DC migration from the plaques.http://dx.doi.org/10.1155/2012/320953 |
| spellingShingle | Thomas Nickel Susanne Pfeiler Claudia Summo Reinhard Kopp Georgios Meimarakis Zeljka Sicic Marius Lambert Korbinian Lackermair Robert David Andres Beiras-Fernandez Ingo Kaczmarek Michael Weis oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21 Mediators of Inflammation |
| title | oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21 |
| title_full | oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21 |
| title_fullStr | oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21 |
| title_full_unstemmed | oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21 |
| title_short | oxLDL Downregulates the Dendritic Cell Homing Factors CCR7 and CCL21 |
| title_sort | oxldl downregulates the dendritic cell homing factors ccr7 and ccl21 |
| url | http://dx.doi.org/10.1155/2012/320953 |
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