Isolating high-quality RNA for RNA-Seq from 10-year-old blood samples

Isolating high-quality RNA for RNA-Seq from 10-year-old blood samples

Abstract There is much interest in analysing RNA, particularly with RNA Sequencing, across both research and diagnostic domains. However, its inherent instability renders it susceptible to degradation. Given the imperative for RNA integrity in such applications, proper storage and biobanking of bloo...

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Bibliographic Details
Main Authors: Charlene Portelli, Elisa Seria, Ritienne Attard, Mitra Barzine, Eva M. Esquinas-Roman, Francesca Borg Carbott, Karen Cassar, Matthew Vella, Brendon P. Scicluna, Jean-Paul Ebejer, Rosienne Farrugia, Stephanie Bezzina Wettinger
Format: Article
Language:English
Published: Nature Portfolio 2024-12-01
Series:Scientific Reports
Subjects:
Biobanking
Transcriptomics
Multi-omics
RNA isolation
Long-term storage
RNA-Seq
Online Access:https://doi.org/10.1038/s41598-024-80287-4
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https://doi.org/10.1038/s41598-024-80287-4

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