Thermal Inactivation of Salmonella, Escherichia coli, and Enterococcus faecium NRRL B-2354 in Pasta Matrices
Limited data are currently available characterizing the thermal resistance of foodborne pathogens in semolina flour and intermediate pasta matrices representative of commercial conditions during processing. Pasta producers require these data to be compliant with federal regulations since Salmonella...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/jfq/8881133 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832575536341188608 |
|---|---|
| author | Kristi S. Gowans Bradley J. Taylor Brad D. Geary Richard A. Robison Gene J. Ahlborn |
| author_facet | Kristi S. Gowans Bradley J. Taylor Brad D. Geary Richard A. Robison Gene J. Ahlborn |
| author_sort | Kristi S. Gowans |
| collection | DOAJ |
| description | Limited data are currently available characterizing the thermal resistance of foodborne pathogens in semolina flour and intermediate pasta matrices representative of commercial conditions during processing. Pasta producers require these data to be compliant with federal regulations since Salmonella spp. and Escherichia coli demonstrate survival in flour and dried pasta products. This study investigated the heat resistance of Salmonella, pathogenic E. coli, and E. faecium NRRL B-2354 in semolina flour and partially dried pasta intermediates via thermal death time (TDT) studies. The appropriateness of E. faecium NRRL B-2354 as a surrogate in semolina flour and pasta matrices was determined. Inoculated pasta matrices equilibrated to target water activities of 0.85, 0.88, and 0.91 (at 25°C) underwent isothermal inactivation treatments at 65°C, 70°C, and 75°C. Serial dilution and direct plating methods allowed for the estimation of bacterial survival at each treatment. In representative pasta matrices, the D values for each microorganism increased as water activity decreased from 0.91 to 0.85. Surprisingly, Salmonella and E. coli did not exhibit significantly different thermal resistance in pasta. The greatest heat resistance was seen in semolina flour (aw 0.45). E. faecium was significantly more thermal resistant than both pathogens in all treatments when the temperature was ≤ 70°C. The results show that E. faecium strain NRRL B-2354 is not an ideal proxy for Salmonella and E. coli in semolina and pasta matrices. Analysis of the TDT data also found that a long-goods pasta drying process can achieve ≥ 7-log reductions of Salmonella and E. coli when following good manufacturing practices. |
| format | Article |
| id | doaj-art-994a705e784a4c04bc923acecfdb814f |
| institution | Kabale University |
| issn | 1745-4557 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-994a705e784a4c04bc923acecfdb814f2025-02-01T00:00:04ZengWileyJournal of Food Quality1745-45572025-01-01202510.1155/jfq/8881133Thermal Inactivation of Salmonella, Escherichia coli, and Enterococcus faecium NRRL B-2354 in Pasta MatricesKristi S. Gowans0Bradley J. Taylor1Brad D. Geary2Richard A. Robison3Gene J. Ahlborn4Department of Nutrition, Dietetics, and Food ScienceDepartment of Nutrition, Dietetics, and Food ScienceDepartment of Plant and Wildlife ScienceDepartment of Microbiology and Molecular BiologyDepartment of Nutrition, Dietetics, and Food ScienceLimited data are currently available characterizing the thermal resistance of foodborne pathogens in semolina flour and intermediate pasta matrices representative of commercial conditions during processing. Pasta producers require these data to be compliant with federal regulations since Salmonella spp. and Escherichia coli demonstrate survival in flour and dried pasta products. This study investigated the heat resistance of Salmonella, pathogenic E. coli, and E. faecium NRRL B-2354 in semolina flour and partially dried pasta intermediates via thermal death time (TDT) studies. The appropriateness of E. faecium NRRL B-2354 as a surrogate in semolina flour and pasta matrices was determined. Inoculated pasta matrices equilibrated to target water activities of 0.85, 0.88, and 0.91 (at 25°C) underwent isothermal inactivation treatments at 65°C, 70°C, and 75°C. Serial dilution and direct plating methods allowed for the estimation of bacterial survival at each treatment. In representative pasta matrices, the D values for each microorganism increased as water activity decreased from 0.91 to 0.85. Surprisingly, Salmonella and E. coli did not exhibit significantly different thermal resistance in pasta. The greatest heat resistance was seen in semolina flour (aw 0.45). E. faecium was significantly more thermal resistant than both pathogens in all treatments when the temperature was ≤ 70°C. The results show that E. faecium strain NRRL B-2354 is not an ideal proxy for Salmonella and E. coli in semolina and pasta matrices. Analysis of the TDT data also found that a long-goods pasta drying process can achieve ≥ 7-log reductions of Salmonella and E. coli when following good manufacturing practices.http://dx.doi.org/10.1155/jfq/8881133 |
| spellingShingle | Kristi S. Gowans Bradley J. Taylor Brad D. Geary Richard A. Robison Gene J. Ahlborn Thermal Inactivation of Salmonella, Escherichia coli, and Enterococcus faecium NRRL B-2354 in Pasta Matrices Journal of Food Quality |
| title | Thermal Inactivation of Salmonella, Escherichia coli, and Enterococcus faecium NRRL B-2354 in Pasta Matrices |
| title_full | Thermal Inactivation of Salmonella, Escherichia coli, and Enterococcus faecium NRRL B-2354 in Pasta Matrices |
| title_fullStr | Thermal Inactivation of Salmonella, Escherichia coli, and Enterococcus faecium NRRL B-2354 in Pasta Matrices |
| title_full_unstemmed | Thermal Inactivation of Salmonella, Escherichia coli, and Enterococcus faecium NRRL B-2354 in Pasta Matrices |
| title_short | Thermal Inactivation of Salmonella, Escherichia coli, and Enterococcus faecium NRRL B-2354 in Pasta Matrices |
| title_sort | thermal inactivation of salmonella escherichia coli and enterococcus faecium nrrl b 2354 in pasta matrices |
| url | http://dx.doi.org/10.1155/jfq/8881133 |
| work_keys_str_mv | AT kristisgowans thermalinactivationofsalmonellaescherichiacoliandenterococcusfaeciumnrrlb2354inpastamatrices AT bradleyjtaylor thermalinactivationofsalmonellaescherichiacoliandenterococcusfaeciumnrrlb2354inpastamatrices AT braddgeary thermalinactivationofsalmonellaescherichiacoliandenterococcusfaeciumnrrlb2354inpastamatrices AT richardarobison thermalinactivationofsalmonellaescherichiacoliandenterococcusfaeciumnrrlb2354inpastamatrices AT genejahlborn thermalinactivationofsalmonellaescherichiacoliandenterococcusfaeciumnrrlb2354inpastamatrices |