Prognostic significance of CDK1 expression in diffuse large B-Cell lymphoma
Abstract Background Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma in adult, characterized by uncontrolled cell proliferation and strong aggressiveness. Previous studies have found that cyclin-dependent kinase 1(CDK1) are related to tumor growth and metastasis. However, the role o...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12885-024-13388-y |
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| author | Qiuni Chen Lei Xu Chuanyang Lu Yujie Xue Xue Gong Yuye Shi Chunling Wang Liang Yu |
| author_facet | Qiuni Chen Lei Xu Chuanyang Lu Yujie Xue Xue Gong Yuye Shi Chunling Wang Liang Yu |
| author_sort | Qiuni Chen |
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| description | Abstract Background Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma in adult, characterized by uncontrolled cell proliferation and strong aggressiveness. Previous studies have found that cyclin-dependent kinase 1(CDK1) are related to tumor growth and metastasis. However, the role of CDK1 in DLBCL is exclusive. This study investigated the clinical implications and expression of CDK1 in DLBCL. Methods Gene expression data for healthy subjects were sourced from the Genotype-Tissue Expression repository. Clinical details and survival statistics of patients with DLBCL were obtained from the Gene Expression Omnibus archive (GSE10846). Patients were categorized based on CDK1 expression levels, and differences in clinical outcomes between the groups were examined. Univariate and multivariate Cox regression analyses were used to ascertain whether CDK1 expression independently predicted DLBCL prognosis. The protein expression of CDK1 was gauged by immunohistochemistry. Additionally, we investigated the effect of CDK1 inhibition on DLBCL cell growth and death using the Cell Counting Kit-8 and flow cytometry. Results In the control group, CDK1 expression was predominantly observed in the hematopoietic and reproductive systems. CDK1 levels in patients with DLBCL were notably elevated compared with those in controls. Significant differences were noted in the lactate dehydrogenase ratio and overall survival based on CDK1 expression. Statistical analyses confirmed that CDK1 was an independent predictor of DLBCL outcomes. Elevated CDK1 protein levels were observed in a significant number of DLBCL samples, in contrast to normal lymph node samples from individuals without lymphoma. The inhibitor Ro-3306 curtails DLBCL cell growth and enhances cell death in vitro. Conclusions Elevated CDK1 levels are correlated with poor prognosis in patients with DLBCL. |
| format | Article |
| id | doaj-art-990435d8ab8848eeb81e92b1424a0162 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Cancer |
| spelling | doaj-art-990435d8ab8848eeb81e92b1424a01622025-01-12T12:27:45ZengBMCBMC Cancer1471-24072025-01-012511910.1186/s12885-024-13388-yPrognostic significance of CDK1 expression in diffuse large B-Cell lymphomaQiuni Chen0Lei Xu1Chuanyang Lu2Yujie Xue3Xue Gong4Yuye Shi5Chunling Wang6Liang Yu7Department of Hematology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Hematology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Hematology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Pathology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Pathology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Hematology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Hematology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityDepartment of Hematology, The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityAbstract Background Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma in adult, characterized by uncontrolled cell proliferation and strong aggressiveness. Previous studies have found that cyclin-dependent kinase 1(CDK1) are related to tumor growth and metastasis. However, the role of CDK1 in DLBCL is exclusive. This study investigated the clinical implications and expression of CDK1 in DLBCL. Methods Gene expression data for healthy subjects were sourced from the Genotype-Tissue Expression repository. Clinical details and survival statistics of patients with DLBCL were obtained from the Gene Expression Omnibus archive (GSE10846). Patients were categorized based on CDK1 expression levels, and differences in clinical outcomes between the groups were examined. Univariate and multivariate Cox regression analyses were used to ascertain whether CDK1 expression independently predicted DLBCL prognosis. The protein expression of CDK1 was gauged by immunohistochemistry. Additionally, we investigated the effect of CDK1 inhibition on DLBCL cell growth and death using the Cell Counting Kit-8 and flow cytometry. Results In the control group, CDK1 expression was predominantly observed in the hematopoietic and reproductive systems. CDK1 levels in patients with DLBCL were notably elevated compared with those in controls. Significant differences were noted in the lactate dehydrogenase ratio and overall survival based on CDK1 expression. Statistical analyses confirmed that CDK1 was an independent predictor of DLBCL outcomes. Elevated CDK1 protein levels were observed in a significant number of DLBCL samples, in contrast to normal lymph node samples from individuals without lymphoma. The inhibitor Ro-3306 curtails DLBCL cell growth and enhances cell death in vitro. Conclusions Elevated CDK1 levels are correlated with poor prognosis in patients with DLBCL.https://doi.org/10.1186/s12885-024-13388-yDiffuse large B-cell lymphomaCDK1BioinformaticsGenotype-tissue expression repositoryGene expression Omnibus archive |
| spellingShingle | Qiuni Chen Lei Xu Chuanyang Lu Yujie Xue Xue Gong Yuye Shi Chunling Wang Liang Yu Prognostic significance of CDK1 expression in diffuse large B-Cell lymphoma BMC Cancer Diffuse large B-cell lymphoma CDK1 Bioinformatics Genotype-tissue expression repository Gene expression Omnibus archive |
| title | Prognostic significance of CDK1 expression in diffuse large B-Cell lymphoma |
| title_full | Prognostic significance of CDK1 expression in diffuse large B-Cell lymphoma |
| title_fullStr | Prognostic significance of CDK1 expression in diffuse large B-Cell lymphoma |
| title_full_unstemmed | Prognostic significance of CDK1 expression in diffuse large B-Cell lymphoma |
| title_short | Prognostic significance of CDK1 expression in diffuse large B-Cell lymphoma |
| title_sort | prognostic significance of cdk1 expression in diffuse large b cell lymphoma |
| topic | Diffuse large B-cell lymphoma CDK1 Bioinformatics Genotype-tissue expression repository Gene expression Omnibus archive |
| url | https://doi.org/10.1186/s12885-024-13388-y |
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