Long PCR-Based Technique for Detection of Transposon Insertions In and Around Cloned Genes of Drosophila melanogaster
A technique to detect a transposable element insertion greater than 5 kb away from a given gene-specific site is described. PCR is performed on genomic DNA isolated from a pool containing one heterozygous mutant fly, carrying an amplifiable allele, within a pool of 100 flies with no amplifiable sequ...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-12-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96216st03 |
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