N-cadherin对视网膜色素上皮细胞增殖及迁移侵袭的影响
【目的】为了明确神经钙黏蛋白(N-cadherin)在糖尿病视网膜病变上皮-间质转化中的作用,探讨N-cadherin对视网膜色素上皮细胞增殖、迁移及侵袭力的影响及作用机制。【方法】分别采用Ad-N-cadherin(Ad-N-cad)、Ad-si N-cadherin(si N-cad)及Ad-GFP、Ad-si Control(si Con)对照腺病毒处理细胞以过表达或沉默N-cadherin,采取葡萄糖(25mmol/L)处理视网膜色素上皮细胞以模拟高糖状态,使用CCK8试剂检测细胞增殖情况,Transwell小室检测细胞的垂直迁移能力及侵袭能力。【结果】Transwell迁移和侵袭实验...
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Format: | Article |
Language: | zho |
Published: |
Editorial Office of Journal of Sun Yat-sen University
2019-01-01
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Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
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Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/44064779/ |
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