Multicenter study correlating molecular characteristics and clinical outcomes of cancer cases with patient-derived organoids
Abstract Background 3D-spatial interaction between cancer cells influences tumor behavior, making it essential to replicate tumor structures for predicting patient outcomes. Methods We collected data from three multicenter prospective studies to evaluate the ability to establish Patient-Derived Orga...
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2025-07-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
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| Online Access: | https://doi.org/10.1186/s13046-025-03437-0 |
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| author | Paloma Navarro Tatiana P. Grazioso Arantzazu Barquín Maria Barba Mónica Yagüe Carlos Millán Irene López Elena Sevillano Miguel Quiralte Paloma Fernández Diego Losada Eduardo Caleiras Julia Calzas Beatriz Jiménez Sergio Ruiz-Llorente Juan Justo Félix Guerrero Vital Hevia Raquel Martín Francisco José Pérez-Rodriguez Julia Tejerina Mario Prieto Paula Comune Juan Francisco Rodriguez-Moreno Jesús García-Donás |
| author_facet | Paloma Navarro Tatiana P. Grazioso Arantzazu Barquín Maria Barba Mónica Yagüe Carlos Millán Irene López Elena Sevillano Miguel Quiralte Paloma Fernández Diego Losada Eduardo Caleiras Julia Calzas Beatriz Jiménez Sergio Ruiz-Llorente Juan Justo Félix Guerrero Vital Hevia Raquel Martín Francisco José Pérez-Rodriguez Julia Tejerina Mario Prieto Paula Comune Juan Francisco Rodriguez-Moreno Jesús García-Donás |
| author_sort | Paloma Navarro |
| collection | DOAJ |
| description | Abstract Background 3D-spatial interaction between cancer cells influences tumor behavior, making it essential to replicate tumor structures for predicting patient outcomes. Methods We collected data from three multicenter prospective studies to evaluate the ability to establish Patient-Derived Organoids (PDOs) from different biological samples and timepoints, correlating their characteristics and drug sensitivity with clinical outcomes. Results From 184 patients (17 tumor types), 249 samples were collected: 149 (59.8%) from tumor tissue, 61 (24.5%) from peritoneal fluids, 39 (15.7%) from peripheral blood. Success rates for PDO establishment were 39.5%, 34.4%, and 25.6%, respectively. PDOs reproduced pathological and immunohistochemical patterns of source tumors, with pathogenic variants confirmed in 84% (21/25). In a series of 13 baseline and sequential PDOs from 9 patients undergoing treatment, responses to therapy mirrored patient responses during therapy. Conclusions PDOs preserve tumor features, reflect disease progression, and predict treatment responses, providing valuable models to complement molecular testing in precision medicine. |
| format | Article |
| id | doaj-art-96791fb5cb5642c7982ab040f6dfce7e |
| institution | Kabale University |
| issn | 1756-9966 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj-art-96791fb5cb5642c7982ab040f6dfce7e2025-08-20T04:01:47ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662025-07-0144112410.1186/s13046-025-03437-0Multicenter study correlating molecular characteristics and clinical outcomes of cancer cases with patient-derived organoidsPaloma Navarro0Tatiana P. Grazioso1Arantzazu Barquín2Maria Barba3Mónica Yagüe4Carlos Millán5Irene López6Elena Sevillano7Miguel Quiralte8Paloma Fernández9Diego Losada10Eduardo Caleiras11Julia Calzas12Beatriz Jiménez13Sergio Ruiz-Llorente14Juan Justo15Félix Guerrero16Vital Hevia17Raquel Martín18Francisco José Pérez-Rodriguez19Julia Tejerina20Mario Prieto21Paula Comune22Juan Francisco Rodriguez-Moreno23Jesús García-Donás24Laboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsLaboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsLaboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsLaboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsLaboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsHM Hospitals Health Research InstituteHM Hospitals Health Research InstituteLaboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsLaboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsInstitute of Applied Molecular Medicine (IMMA), Department of Basic Medical Sciences, Facultad de Medicina, Universidad San Pablo CEU, CEU Universities, Urbanización MontepríncipeLaboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsHistopathology Core Unit, Spanish National Cancer Center (CNIO)Medical Oncology Department, Fuenlabrada University HospitalHM Hospitals Health Research InstituteLaboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsHM Faculty of Health Sciences, Camilo José Cela UniversityDepartment of Urology, HM HospitalesDepartment of Urology, HM HospitalesDepartment of Pathology, Therapeutic Target Laboratory, Hospital Universitario HM SanchinarroDepartment of Pathology, Therapeutic Target Laboratory, Hospital Universitario HM SanchinarroHospital Universitario San JorgeDepartment of Pathology, Therapeutic Target Laboratory, Hospital Universitario HM SanchinarroVivía BiotechLaboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsLaboratory of Innovation in Oncology, Genitourinary, Gynecological and Skin Cancer Unit, HM Clara Campal Comprehensive Cancer Centre (CIOCC) MADRID. Sanchinarro HM University Hospital, HM HospitalsAbstract Background 3D-spatial interaction between cancer cells influences tumor behavior, making it essential to replicate tumor structures for predicting patient outcomes. Methods We collected data from three multicenter prospective studies to evaluate the ability to establish Patient-Derived Organoids (PDOs) from different biological samples and timepoints, correlating their characteristics and drug sensitivity with clinical outcomes. Results From 184 patients (17 tumor types), 249 samples were collected: 149 (59.8%) from tumor tissue, 61 (24.5%) from peritoneal fluids, 39 (15.7%) from peripheral blood. Success rates for PDO establishment were 39.5%, 34.4%, and 25.6%, respectively. PDOs reproduced pathological and immunohistochemical patterns of source tumors, with pathogenic variants confirmed in 84% (21/25). In a series of 13 baseline and sequential PDOs from 9 patients undergoing treatment, responses to therapy mirrored patient responses during therapy. Conclusions PDOs preserve tumor features, reflect disease progression, and predict treatment responses, providing valuable models to complement molecular testing in precision medicine.https://doi.org/10.1186/s13046-025-03437-0Precision MedicinePatient-derived organoidsDrug screening |
| spellingShingle | Paloma Navarro Tatiana P. Grazioso Arantzazu Barquín Maria Barba Mónica Yagüe Carlos Millán Irene López Elena Sevillano Miguel Quiralte Paloma Fernández Diego Losada Eduardo Caleiras Julia Calzas Beatriz Jiménez Sergio Ruiz-Llorente Juan Justo Félix Guerrero Vital Hevia Raquel Martín Francisco José Pérez-Rodriguez Julia Tejerina Mario Prieto Paula Comune Juan Francisco Rodriguez-Moreno Jesús García-Donás Multicenter study correlating molecular characteristics and clinical outcomes of cancer cases with patient-derived organoids Journal of Experimental & Clinical Cancer Research Precision Medicine Patient-derived organoids Drug screening |
| title | Multicenter study correlating molecular characteristics and clinical outcomes of cancer cases with patient-derived organoids |
| title_full | Multicenter study correlating molecular characteristics and clinical outcomes of cancer cases with patient-derived organoids |
| title_fullStr | Multicenter study correlating molecular characteristics and clinical outcomes of cancer cases with patient-derived organoids |
| title_full_unstemmed | Multicenter study correlating molecular characteristics and clinical outcomes of cancer cases with patient-derived organoids |
| title_short | Multicenter study correlating molecular characteristics and clinical outcomes of cancer cases with patient-derived organoids |
| title_sort | multicenter study correlating molecular characteristics and clinical outcomes of cancer cases with patient derived organoids |
| topic | Precision Medicine Patient-derived organoids Drug screening |
| url | https://doi.org/10.1186/s13046-025-03437-0 |
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