A novel strategy for eradication of staphylococcal biofilms using blood clots
IntroductionInfections with coagulase negative staphylococcal species (CoNS) are a major cause of mortality and morbidity in joint and heart valve replacement procedures, largely due to biofilm formation. Cells within biofilms have higher rates of antibiotic resistance than their planktonic counterp...
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| Format: | Article |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1507486/full |
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| author | Kayla E. Grooters Sheridan L. Hayes David M. Richter Jennifer C. Ku Robert Sawyer Yong Li |
| author_facet | Kayla E. Grooters Sheridan L. Hayes David M. Richter Jennifer C. Ku Robert Sawyer Yong Li |
| author_sort | Kayla E. Grooters |
| collection | DOAJ |
| description | IntroductionInfections with coagulase negative staphylococcal species (CoNS) are a major cause of mortality and morbidity in joint and heart valve replacement procedures, largely due to biofilm formation. Cells within biofilms have higher rates of antibiotic resistance than their planktonic counterparts; consequently, novel mechanisms are needed to combat these infections.MethodsTo enhance antibiotic delivery and penetration, this innovative study involved treating CoNS biofilms with murine blood clots impregnated with antibiotics. We then investigated the impact of this treatment on biofilm density, metabolism, and architecture.ResultsOur pilot study demonstrates that this method of antibiotic delivery results in improved biofilm clearance, relative to conventional exposure methods.DiscussionOur results demonstrate that blood clot exposure has an intrinsic impact on biofilm density and potentially reduces colonization, warrenting further investigation into the mechanism. |
| format | Article |
| id | doaj-art-960b5313ae4d4d26ba258123d88f0a00 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-960b5313ae4d4d26ba258123d88f0a002025-01-31T06:40:04ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011510.3389/fcimb.2025.15074861507486A novel strategy for eradication of staphylococcal biofilms using blood clotsKayla E. Grooters0Sheridan L. Hayes1David M. Richter2Jennifer C. Ku3Robert Sawyer4Yong Li5Department of Medicine, Western Michigan University Homer Stryker M.D. School of Medicine, Kalamazoo, MI, United StatesDivision of Medical Engineering, Department of Surgical Science, Western Michigan University Homer Stryker MD School of Medicine, Kalamazoo, MI, United StatesDepartment of Medicine, Western Michigan University Homer Stryker M.D. School of Medicine, Kalamazoo, MI, United StatesDepartment of Medicine, Western Michigan University Homer Stryker M.D. School of Medicine, Kalamazoo, MI, United StatesDivision of Medical Engineering, Department of Surgical Science, Western Michigan University Homer Stryker MD School of Medicine, Kalamazoo, MI, United StatesDivision of Medical Engineering, Department of Surgical Science, Western Michigan University Homer Stryker MD School of Medicine, Kalamazoo, MI, United StatesIntroductionInfections with coagulase negative staphylococcal species (CoNS) are a major cause of mortality and morbidity in joint and heart valve replacement procedures, largely due to biofilm formation. Cells within biofilms have higher rates of antibiotic resistance than their planktonic counterparts; consequently, novel mechanisms are needed to combat these infections.MethodsTo enhance antibiotic delivery and penetration, this innovative study involved treating CoNS biofilms with murine blood clots impregnated with antibiotics. We then investigated the impact of this treatment on biofilm density, metabolism, and architecture.ResultsOur pilot study demonstrates that this method of antibiotic delivery results in improved biofilm clearance, relative to conventional exposure methods.DiscussionOur results demonstrate that blood clot exposure has an intrinsic impact on biofilm density and potentially reduces colonization, warrenting further investigation into the mechanism.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1507486/fullbiofilmantibiotic resistancestaphylococciprosthetic joint infectionsbiomaterials |
| spellingShingle | Kayla E. Grooters Sheridan L. Hayes David M. Richter Jennifer C. Ku Robert Sawyer Yong Li A novel strategy for eradication of staphylococcal biofilms using blood clots Frontiers in Cellular and Infection Microbiology biofilm antibiotic resistance staphylococci prosthetic joint infections biomaterials |
| title | A novel strategy for eradication of staphylococcal biofilms using blood clots |
| title_full | A novel strategy for eradication of staphylococcal biofilms using blood clots |
| title_fullStr | A novel strategy for eradication of staphylococcal biofilms using blood clots |
| title_full_unstemmed | A novel strategy for eradication of staphylococcal biofilms using blood clots |
| title_short | A novel strategy for eradication of staphylococcal biofilms using blood clots |
| title_sort | novel strategy for eradication of staphylococcal biofilms using blood clots |
| topic | biofilm antibiotic resistance staphylococci prosthetic joint infections biomaterials |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1507486/full |
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