Optimizing Microneutralization and IFN-γ ELISPOT Assays to Evaluate Mpox Immunity

Optimizing Microneutralization and IFN-γ ELISPOT Assays to Evaluate Mpox Immunity

Background: Available assays to measure pox virus neutralizing antibody titers are laborious and take up to 5 days. In addition, assays to measure T cell responses require the use of specific antigens, which may not be the same for all pox viruses. This study reports the development of robust assays...

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Bibliographic Details
Main Authors: Yinyi Yu, Krystal Meza, Chase Colbert, Daniel F. Hoft, Anna Jaunarajs, Azra Blazevic, Sharon E. Frey, Getahun Abate
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Vaccines
Subjects:
mpox
vaccinia
FRNT
microneutralization
PRNT
IFN-γ ELISPOT
Online Access:https://www.mdpi.com/2076-393X/13/1/27
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https://www.mdpi.com/2076-393X/13/1/27

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