Visualization of global RNA synthesis in a human (mini-) organ in situ by click chemistry

Visualization of global RNA synthesis in a human (mini-) organ in situ by click chemistry

RNA synthesis can be detected by 5-ethynyl uridine (EU) incorporation and click chemistry. Despite identifying a fundamental functional process, this technique has yet to be widely applied to complex human tissue systems. By incorporating EU into human hair follicle (HF) organs cultured ex vivo, nas...

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Bibliographic Details
Main Authors: Talveen S Purba, Jack Marsh, Kayumba Ng'andu, Svitlana Kurinna, Ralf Paus
Format: Article
Language:English
Published: Taylor & Francis Group 2018-08-01
Series:BioTechniques
Subjects:
5-ethynyl uridine
click chemistry
human hair follicle
O-propargyl-puromycin
protein synthesis
RNA synthesis
Online Access:https://www.future-science.com/doi/10.2144/btn-2018-0025
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https://www.future-science.com/doi/10.2144/btn-2018-0025

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