Construction of LEU1 gene deleted Saccharomyces cerevisiae based on CRISPR-Cas9 system for brewing low degree of drunkenness rice wine
In order to explore the effect of the deletion of LEU1 gene of isopropyl malate synthase in Saccharomyces cerevisiae on the production of higher alcohols in rice wine brewing, the recombinant plasmids p414-Cas9-BleoR and p426-gRNA. LEU1-kanMX were constructed and electrotransformed into S. cerevisia...
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Department of China Brewing
2024-04-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-4-62.pdf |
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