Random priming PCR strategy to amplify and clone trace amounts of DNA
Here we report a new methodology to study trace amounts of DNA of unknown sequence using a two-step PCR strategy to amplify and clone target DNA. The first PCR is carried out with a partial random primer comprised of a specific 21-nucleotide 5′ sequence, a random heptamer, and a 3′ TGGC clamp. The s...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2003-10-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/03354st06 |
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