Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing
This study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfect...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | CyTA - Journal of Food |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19476337.2024.2351906 |
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| author | Miao He Lingyan Ma Wentao Lv Yingping Xiao Hua Yang Xiaofan Wang Jiangchao Zhao Hangzhen Lan Wen Wang Xingning Xiao |
| author_facet | Miao He Lingyan Ma Wentao Lv Yingping Xiao Hua Yang Xiaofan Wang Jiangchao Zhao Hangzhen Lan Wen Wang Xingning Xiao |
| author_sort | Miao He |
| collection | DOAJ |
| description | This study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfectant was assessed by comparing the bacterial communities in the viable samples before and after disinfection. The bacterial abundances in meat, chopping boards, and knives were positively correlated (r > 0.95, p < .05), suggesting potential cross-contamination risks during market transactions. The species richness indices for the external transport vehicles, pork hooks, and floors were significantly different from those of the samples taken after decontamination (p < .05). Linear discriminant analysis Effect Size (LEfSe) analyses showed that Firmicutes, Proteobacteria, and Chloroflexi were significantly more abundant in the transaction period, whereas Bacteroidetes levels decreased compared to the clean-up period. NaClO disinfection altered the composition of bacterial communities. |
| format | Article |
| id | doaj-art-9426f62a29e045029ffbcef331275cbf |
| institution | Kabale University |
| issn | 1947-6337 1947-6345 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | CyTA - Journal of Food |
| spelling | doaj-art-9426f62a29e045029ffbcef331275cbf2024-12-18T13:34:51ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452024-12-0122110.1080/19476337.2024.2351906Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencingMiao He0Lingyan Ma1Wentao Lv2Yingping Xiao3Hua Yang4Xiaofan Wang5Jiangchao Zhao6Hangzhen Lan7Wen Wang8Xingning Xiao9State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, MOA Laboratory of Quality & Safety Risk Assessment for Agro-products (Hangzhou), Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, MOA Laboratory of Quality & Safety Risk Assessment for Agro-products (Hangzhou), Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, MOA Laboratory of Quality & Safety Risk Assessment for Agro-products (Hangzhou), Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, MOA Laboratory of Quality & Safety Risk Assessment for Agro-products (Hangzhou), Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaDepartment of Animal Science, Division of Agriculture, University of Arkansas, Fayetteville, Arkansas, USADepartment of Animal Science, Division of Agriculture, University of Arkansas, Fayetteville, Arkansas, USAState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, MOA Laboratory of Quality & Safety Risk Assessment for Agro-products (Hangzhou), Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, MOA Laboratory of Quality & Safety Risk Assessment for Agro-products (Hangzhou), Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, ChinaThis study has employed 16S rDNA analysis to elucidate the characteristics of the microbial community in the surroundings, facilities, and meat samples collected from a pork wholesale market with the aim of revealing bacterial contamination. In addition, the efficacy of sodium hypochlorite disinfectant was assessed by comparing the bacterial communities in the viable samples before and after disinfection. The bacterial abundances in meat, chopping boards, and knives were positively correlated (r > 0.95, p < .05), suggesting potential cross-contamination risks during market transactions. The species richness indices for the external transport vehicles, pork hooks, and floors were significantly different from those of the samples taken after decontamination (p < .05). Linear discriminant analysis Effect Size (LEfSe) analyses showed that Firmicutes, Proteobacteria, and Chloroflexi were significantly more abundant in the transaction period, whereas Bacteroidetes levels decreased compared to the clean-up period. NaClO disinfection altered the composition of bacterial communities.https://www.tandfonline.com/doi/10.1080/19476337.2024.2351906Porkwholesaler16S rDNA amplicon sequencingbacterial contaminationNaClO disinfection |
| spellingShingle | Miao He Lingyan Ma Wentao Lv Yingping Xiao Hua Yang Xiaofan Wang Jiangchao Zhao Hangzhen Lan Wen Wang Xingning Xiao Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing CyTA - Journal of Food Pork wholesaler 16S rDNA amplicon sequencing bacterial contamination NaClO disinfection |
| title | Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing |
| title_full | Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing |
| title_fullStr | Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing |
| title_full_unstemmed | Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing |
| title_short | Revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16S rDNA sequencing |
| title_sort | revealing microbial community composition and disinfection efficacy evaluation in wholesale pork markets through 16s rdna sequencing |
| topic | Pork wholesaler 16S rDNA amplicon sequencing bacterial contamination NaClO disinfection |
| url | https://www.tandfonline.com/doi/10.1080/19476337.2024.2351906 |
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