Features of “All LNA” Duplexes Showing a New Type of Nucleic Acid Geometry
“Locked nucleic acids” (LNAs) belong to the backbone-modified nucleic acid family. The 2′-O,4′-C-methylene-β-D-ribofuranose nucleotides are used for single or multiple substitutions in RNA molecules and thereby introduce enhanced bio- and thermostability. This renders LNAs powerful tools for diagnos...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.1155/2012/156035 |
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| author | Charlotte Förster André Eichert Dominik Oberthür Christian Betzel Reinhard Geßner Andreas Nitsche Jens P. Fürste |
| author_facet | Charlotte Förster André Eichert Dominik Oberthür Christian Betzel Reinhard Geßner Andreas Nitsche Jens P. Fürste |
| author_sort | Charlotte Förster |
| collection | DOAJ |
| description | “Locked nucleic acids” (LNAs) belong to the backbone-modified nucleic acid family. The 2′-O,4′-C-methylene-β-D-ribofuranose nucleotides are used for single or multiple substitutions in RNA molecules and thereby introduce enhanced bio- and thermostability. This renders LNAs powerful tools for diagnostic and therapeutic applications. RNA molecules maintain the overall canonical A-type conformation upon substitution of single or multiple residues/nucleotides by LNA monomers. The structures of “all” LNA homoduplexes, however, exhibit significant differences in their overall geometry, in particular a decreased twist, roll and propeller twist. This results in a widening of the major groove, a decrease in helical winding, and an enlarged helical pitch. Therefore, the LNA duplex structure can no longer be described as a canonical A-type RNA geometry but can rather be brought into proximity to other backbone-modified nucleic acids, like glycol nucleic acids or peptide nucleic acids. LNA-modified nucleic acids provide thus structural and functional features that may be successfully exploited for future application in biotechnology and drug discovery. |
| format | Article |
| id | doaj-art-93ee7fccbafb4a209a2d2b9c6ad3ff93 |
| institution | Kabale University |
| issn | 2090-0201 2090-021X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-93ee7fccbafb4a209a2d2b9c6ad3ff932025-02-03T05:44:16ZengWileyJournal of Nucleic Acids2090-02012090-021X2012-01-01201210.1155/2012/156035156035Features of “All LNA” Duplexes Showing a New Type of Nucleic Acid GeometryCharlotte Förster0André Eichert1Dominik Oberthür2Christian Betzel3Reinhard Geßner4Andreas Nitsche5Jens P. Fürste6Institut für Chemie und Biochemie, Freie Universität Berlin, 14195 Berlin, GermanyInstitut für Chemie und Biochemie, Freie Universität Berlin, 14195 Berlin, GermanyLaboratory for Structural Biology of Infection and Inflammation, Institute of Biochemistry and Molecular Biology, University of Hamburg, c/o DESY, 22603 Hamburg, GermanyLaboratory for Structural Biology of Infection and Inflammation, Institute of Biochemistry and Molecular Biology, University of Hamburg, c/o DESY, 22603 Hamburg, GermanyChirurgische klinik II—Visceral, Transplantations-, Thorax- und Gefäßchirurgie, Universitätsklinik Leipzig, Liebigstraße 20, 04103 Leipzig, GermanyZentrum für Biologische Sicherheit 1, Robert Koch Institut, Nordufer 20, 13353 Berlin, GermanyInstitut für Chemie und Biochemie, Freie Universität Berlin, 14195 Berlin, Germany“Locked nucleic acids” (LNAs) belong to the backbone-modified nucleic acid family. The 2′-O,4′-C-methylene-β-D-ribofuranose nucleotides are used for single or multiple substitutions in RNA molecules and thereby introduce enhanced bio- and thermostability. This renders LNAs powerful tools for diagnostic and therapeutic applications. RNA molecules maintain the overall canonical A-type conformation upon substitution of single or multiple residues/nucleotides by LNA monomers. The structures of “all” LNA homoduplexes, however, exhibit significant differences in their overall geometry, in particular a decreased twist, roll and propeller twist. This results in a widening of the major groove, a decrease in helical winding, and an enlarged helical pitch. Therefore, the LNA duplex structure can no longer be described as a canonical A-type RNA geometry but can rather be brought into proximity to other backbone-modified nucleic acids, like glycol nucleic acids or peptide nucleic acids. LNA-modified nucleic acids provide thus structural and functional features that may be successfully exploited for future application in biotechnology and drug discovery.http://dx.doi.org/10.1155/2012/156035 |
| spellingShingle | Charlotte Förster André Eichert Dominik Oberthür Christian Betzel Reinhard Geßner Andreas Nitsche Jens P. Fürste Features of “All LNA” Duplexes Showing a New Type of Nucleic Acid Geometry Journal of Nucleic Acids |
| title | Features of “All LNA” Duplexes Showing a New Type of Nucleic Acid Geometry |
| title_full | Features of “All LNA” Duplexes Showing a New Type of Nucleic Acid Geometry |
| title_fullStr | Features of “All LNA” Duplexes Showing a New Type of Nucleic Acid Geometry |
| title_full_unstemmed | Features of “All LNA” Duplexes Showing a New Type of Nucleic Acid Geometry |
| title_short | Features of “All LNA” Duplexes Showing a New Type of Nucleic Acid Geometry |
| title_sort | features of all lna duplexes showing a new type of nucleic acid geometry |
| url | http://dx.doi.org/10.1155/2012/156035 |
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