Common cell lysis procedures distort ribosome profiling analyses of gene expression

Common cell lysis procedures distort ribosome profiling analyses of gene expression

Abstract Ribosome profiling is a powerful technique used to study gene expression on a transcriptome-wide scale. It involves sequencing of mRNA fragments protected by ribosomes from ribonuclease digestion. The initial steps commonly involve cell lysis followed by centrifugation and ribonuclease dige...

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Bibliographic Details
Main Authors: Aoife O’Connell, Alla D. Fedorova, Patrick B. F. O’Connor, Alexander V. Zhdanov, Pavel V. Baranov, Gary Loughran, Dmitry E. Andreev
Format: Article
Language:English
Published: BMC 2025-08-01
Series:Genome Biology
Subjects:
Ribosome profiling
Polysome profiling
Gene expression
Riboseq
Cytoskeleton
Insoluble fraction
Online Access:https://doi.org/10.1186/s13059-025-03651-1
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https://doi.org/10.1186/s13059-025-03651-1

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