Use of CMFDA and CMTMR Fluorescent Dyes in FACS®-Based Antibody Screening
Cell-based immunizations are often used when membrane antigens are difficult to purify. To confirm that an antibody binding to the surface of a cell line is, in fact, binding to the desired antigen, FACS® can be performed independently on two cell lines, a transfected cell line expressing the antige...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2002-03-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/02323dd05 |
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