Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration
Aim: Evaluate the selective cytotoxic effect of the palindromic sequence RWQWRWQWR and its analogues obtained by replacement of L-amino acids by D-amino acids or the functionalization by adding the RGD (integrin ligand motif) to the peptide. Methods: Peptides were obtained by SPPS, characterized by...
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| Format: | Article |
| Language: | English |
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Open Exploration
2024-07-01
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| Series: | Exploration of Drug Science |
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| Online Access: | https://www.explorationpub.com/uploads/Article/A100852/100852.pdf |
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| author | Andrea Barragán-Cárdenas Daniel Castellar-Almonacid Yerly Vargas-Casanova Claudia Parra-Giraldo Adriana Umaña-Pérez Joel López-Meza Zuly Rivera-Monroy Javier García-Castañeda |
| author_facet | Andrea Barragán-Cárdenas Daniel Castellar-Almonacid Yerly Vargas-Casanova Claudia Parra-Giraldo Adriana Umaña-Pérez Joel López-Meza Zuly Rivera-Monroy Javier García-Castañeda |
| author_sort | Andrea Barragán-Cárdenas |
| collection | DOAJ |
| description | Aim: Evaluate the selective cytotoxic effect of the palindromic sequence RWQWRWQWR and its analogues obtained by replacement of L-amino acids by D-amino acids or the functionalization by adding the RGD (integrin ligand motif) to the peptide. Methods: Peptides were obtained by SPPS, characterized by RP-HPLC and ESI-QTOF and its biological activity was evaluated using MTT assays. Evaluation of mechanism associated to the cytotoxic effect were carried out by flow cytometry, RT-qPCR, wound healing, transwell and zymography. Results: The peptides with replacements of D-amino acid showed a lesser cytotoxic effect against breast cancer cell lines, regardless it was one or several residues modified which suggested a possible specific interaction between the peptide and the cancer cell membrane besides its initial electrostatically contact. On the other hand, addition of the RGD sequence to the palindromic peptide in the N-terminal end resulted in a greater cytotoxic effect against cell lines derived from the three mainly diagnosed breast cancer molecular subtypes. An approximation on mechanisms associated to this effect was evaluated on MCF-7 cells, it shows that the peptide induced apoptosis by activating intrinsic and extrinsic pathway, which correlates with the possibility of a specific interaction, and induces mitochondrial depolarization with release of oxygen reactive species. Also, this peptide induces a reduction in migration and invasion associated with a diminish in metalloprotease 9 activity and a lesser release of IL-6, IL-10 and arginase cytokines. Conclusions: Our results suggest that this promising peptide can be considered for preclinical evaluation in the development of drugs to treat breast cancer and thus mitigate the impact of this disease. |
| format | Article |
| id | doaj-art-924d26737774467a9fed82c2c0fc04cb |
| institution | Kabale University |
| issn | 2836-7677 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Open Exploration |
| record_format | Article |
| series | Exploration of Drug Science |
| spelling | doaj-art-924d26737774467a9fed82c2c0fc04cb2025-02-08T03:30:12ZengOpen ExplorationExploration of Drug Science2836-76772024-07-012436938810.37349/eds.2024.00052Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migrationAndrea Barragán-Cárdenas0https://orcid.org/0000-0001-7545-8129Daniel Castellar-Almonacid1https://orcid.org/0009-0005-1704-2842Yerly Vargas-Casanova2https://orcid.org/0000-0003-4788-8150Claudia Parra-Giraldo3https://orcid.org/0000-0003-1302-5429Adriana Umaña-Pérez4https://orcid.org/0000-0001-9262-2699Joel López-Meza5https://orcid.org/0000-0002-3269-9202Zuly Rivera-Monroy6https://orcid.org/0000-0001-6915-8488Javier García-Castañeda7https://orcid.org/0000-0001-6882-4397Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá 111321, ColombiaDepartamento de Farmacia, Universidad Nacional de Colombia, Bogotá 111321, ColombiaDepartment of Microbiology, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotá 110231, ColombiaDepartment of Microbiology, Faculty of Sciences, Pontificia Universidad Javeriana, Bogotá 110231, ColombiaDepartamento de Química, Universidad Nacional de Colombia, Bogotá 111321, ColombiaCentro Multidisciplinario de Estudios en Biotecnología - Universidad Michoacana de San Nicolás de Hidalgo, Morelia 58030, MéxicoDepartamento de Química, Universidad Nacional de Colombia, Bogotá 111321, ColombiaDepartamento de Farmacia, Universidad Nacional de Colombia, Bogotá 111321, ColombiaAim: Evaluate the selective cytotoxic effect of the palindromic sequence RWQWRWQWR and its analogues obtained by replacement of L-amino acids by D-amino acids or the functionalization by adding the RGD (integrin ligand motif) to the peptide. Methods: Peptides were obtained by SPPS, characterized by RP-HPLC and ESI-QTOF and its biological activity was evaluated using MTT assays. Evaluation of mechanism associated to the cytotoxic effect were carried out by flow cytometry, RT-qPCR, wound healing, transwell and zymography. Results: The peptides with replacements of D-amino acid showed a lesser cytotoxic effect against breast cancer cell lines, regardless it was one or several residues modified which suggested a possible specific interaction between the peptide and the cancer cell membrane besides its initial electrostatically contact. On the other hand, addition of the RGD sequence to the palindromic peptide in the N-terminal end resulted in a greater cytotoxic effect against cell lines derived from the three mainly diagnosed breast cancer molecular subtypes. An approximation on mechanisms associated to this effect was evaluated on MCF-7 cells, it shows that the peptide induced apoptosis by activating intrinsic and extrinsic pathway, which correlates with the possibility of a specific interaction, and induces mitochondrial depolarization with release of oxygen reactive species. Also, this peptide induces a reduction in migration and invasion associated with a diminish in metalloprotease 9 activity and a lesser release of IL-6, IL-10 and arginase cytokines. Conclusions: Our results suggest that this promising peptide can be considered for preclinical evaluation in the development of drugs to treat breast cancer and thus mitigate the impact of this disease.https://www.explorationpub.com/uploads/Article/A100852/100852.pdfbreast canceranticancer peptidesapoptosismigration |
| spellingShingle | Andrea Barragán-Cárdenas Daniel Castellar-Almonacid Yerly Vargas-Casanova Claudia Parra-Giraldo Adriana Umaña-Pérez Joel López-Meza Zuly Rivera-Monroy Javier García-Castañeda Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration Exploration of Drug Science breast cancer anticancer peptides apoptosis migration |
| title | Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration |
| title_full | Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration |
| title_fullStr | Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration |
| title_full_unstemmed | Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration |
| title_short | Enhanced breast cancer cell targeting: RGD integrin ligand potentiates RWQWRWQWR’s cytotoxicity and inhibits migration |
| title_sort | enhanced breast cancer cell targeting rgd integrin ligand potentiates rwqwrwqwr s cytotoxicity and inhibits migration |
| topic | breast cancer anticancer peptides apoptosis migration |
| url | https://www.explorationpub.com/uploads/Article/A100852/100852.pdf |
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