Evaluation of Acute and Sub-Chronic Toxicity of Lactobacillus rhamnosus GG in Sprague-Dawley Rats
Background: Probiotic-based bacteriotherapy has emerged as a potentially effective strategy for preventing infectious diseases. Lactobacillus strains consumed as probiotics and the safety of these spp. has been questioned due to reported unexpected responses. Hence, the present study has been conduc...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Advanced Biomedical Research |
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| Online Access: | https://journals.lww.com/10.4103/abr.abr_13_24 |
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| author | Vedam Venkata Kanthi Vaishnavi Urmila Banik Gokul Shankar Sabesan Arun K. Adhikary Subramani Parasuraman |
| author_facet | Vedam Venkata Kanthi Vaishnavi Urmila Banik Gokul Shankar Sabesan Arun K. Adhikary Subramani Parasuraman |
| author_sort | Vedam Venkata Kanthi Vaishnavi |
| collection | DOAJ |
| description | Background:
Probiotic-based bacteriotherapy has emerged as a potentially effective strategy for preventing infectious diseases. Lactobacillus strains consumed as probiotics and the safety of these spp. has been questioned due to reported unexpected responses. Hence, the present study has been conducted to evaluate the acute and sub-chronic toxicity of Lactobacillus rhamnosus GG in Sprague-Dawley (SD) rats.
Materials and Methods:
The acute and sub-chronic toxicity effect of L. rhamnosus is studied in rats as per the Organization for Economic Cooperation and Development (OECD), test guideline 423 and 407, respectively.
Results:
In acute toxicity, L. rhamnosus at 1 × 107, 1 × 108, 1 × 109, and 1 × 1010 CFU/mL don’t show any toxic signs. In sub-chronic toxicity, L. rhamnosus at 1 × 106,1 × 107 and 1 × 108 CFU/mL dosages showed dose-depended changes in biochemical and haematological parameters. In this study, one male and one female rat administered with 1 x 108 CFU/mL of L. rhamnosus showed mortality on days 16 and 26, respectively. The animals administered with L. rhamnosus showed no histological changes in the organs such as heart, liver and kidney.
Conclusion:
L. rhamnosus exhibited mild-to-moderate toxic effects at the dose levels of 1 × 106 CFU/mL, 1 × 107 and 1 × 108 CFU/mL in rats. |
| format | Article |
| id | doaj-art-9143f2af77dc4a3fb139c82fd588017c |
| institution | Kabale University |
| issn | 2277-9175 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj-art-9143f2af77dc4a3fb139c82fd588017c2025-01-08T13:15:59ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752024-12-0113112612610.4103/abr.abr_13_24Evaluation of Acute and Sub-Chronic Toxicity of Lactobacillus rhamnosus GG in Sprague-Dawley RatsVedam Venkata Kanthi VaishnaviUrmila BanikGokul Shankar SabesanArun K. AdhikarySubramani ParasuramanBackground: Probiotic-based bacteriotherapy has emerged as a potentially effective strategy for preventing infectious diseases. Lactobacillus strains consumed as probiotics and the safety of these spp. has been questioned due to reported unexpected responses. Hence, the present study has been conducted to evaluate the acute and sub-chronic toxicity of Lactobacillus rhamnosus GG in Sprague-Dawley (SD) rats. Materials and Methods: The acute and sub-chronic toxicity effect of L. rhamnosus is studied in rats as per the Organization for Economic Cooperation and Development (OECD), test guideline 423 and 407, respectively. Results: In acute toxicity, L. rhamnosus at 1 × 107, 1 × 108, 1 × 109, and 1 × 1010 CFU/mL don’t show any toxic signs. In sub-chronic toxicity, L. rhamnosus at 1 × 106,1 × 107 and 1 × 108 CFU/mL dosages showed dose-depended changes in biochemical and haematological parameters. In this study, one male and one female rat administered with 1 x 108 CFU/mL of L. rhamnosus showed mortality on days 16 and 26, respectively. The animals administered with L. rhamnosus showed no histological changes in the organs such as heart, liver and kidney. Conclusion: L. rhamnosus exhibited mild-to-moderate toxic effects at the dose levels of 1 × 106 CFU/mL, 1 × 107 and 1 × 108 CFU/mL in rats.https://journals.lww.com/10.4103/abr.abr_13_24lactobacillus rhamnosusprobioticstoxicity |
| spellingShingle | Vedam Venkata Kanthi Vaishnavi Urmila Banik Gokul Shankar Sabesan Arun K. Adhikary Subramani Parasuraman Evaluation of Acute and Sub-Chronic Toxicity of Lactobacillus rhamnosus GG in Sprague-Dawley Rats Advanced Biomedical Research lactobacillus rhamnosus probiotics toxicity |
| title | Evaluation of Acute and Sub-Chronic Toxicity of Lactobacillus rhamnosus GG in Sprague-Dawley Rats |
| title_full | Evaluation of Acute and Sub-Chronic Toxicity of Lactobacillus rhamnosus GG in Sprague-Dawley Rats |
| title_fullStr | Evaluation of Acute and Sub-Chronic Toxicity of Lactobacillus rhamnosus GG in Sprague-Dawley Rats |
| title_full_unstemmed | Evaluation of Acute and Sub-Chronic Toxicity of Lactobacillus rhamnosus GG in Sprague-Dawley Rats |
| title_short | Evaluation of Acute and Sub-Chronic Toxicity of Lactobacillus rhamnosus GG in Sprague-Dawley Rats |
| title_sort | evaluation of acute and sub chronic toxicity of lactobacillus rhamnosus gg in sprague dawley rats |
| topic | lactobacillus rhamnosus probiotics toxicity |
| url | https://journals.lww.com/10.4103/abr.abr_13_24 |
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