Effects of Fucoidan on the Inhibition of Biofilm Formation of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Serovar <i>Typhimurium</i> on Seafoods and Its Molecular Antibiofilm Mechanisms
Foodborne illnesses, particularly those caused by <i>Salmonella enterica</i> subsp. <i>enterica</i> Serovar <i>Typhimurium</i>, present a significant challenge to public health, especially within the seafood industry due to biofilm formation on foods. This study i...
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2025-04-01
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| author | Anamika Roy Pantu Kumar Roy Sung Rae Cho Shin Young Park |
| author_facet | Anamika Roy Pantu Kumar Roy Sung Rae Cho Shin Young Park |
| author_sort | Anamika Roy |
| collection | DOAJ |
| description | Foodborne illnesses, particularly those caused by <i>Salmonella enterica</i> subsp. <i>enterica</i> Serovar <i>Typhimurium</i>, present a significant challenge to public health, especially within the seafood industry due to biofilm formation on foods. This study investigated the antibiofilm potential of fucoidan, a sulfated polysaccharide, against <i>Salmonella enterica</i> subsp. <i>enterica</i> Serovar <i>Typhimurium</i> biofilm on crab and shrimp surfaces. Fucoidan’s minimum inhibitory concentration (MIC) was determined to be 150 µg/mL. Sub-MIC (1/8, 1/4, 1/2, and MIC) were evaluated for their impact on inhibition of biofilm formation. Fucoidan treatment resulted in significant, dose-dependent inhibition in biofilm formation, achieving 2.61 log CFU/cm<sup>2</sup> and 2.45 log CFU/cm<sup>2</sup> reductions on crab and shrimp surfaces, respectively. FE-SEM analysis confirmed biofilm disruption and cell membrane damage. Real-time PCR showed the downregulation of quorum-sensing (<i>luxS</i>) and virulence (<i>rpoS</i>, <i>avrA</i>, and <i>hilA</i>) genes. These results propose that fucoidan has the ability as a natural antibacterial agent for controlling <i>Salmonella enterica</i> subsp. <i>enterica</i> Serovar <i>Typhimurium</i> biofilms in seafood processing, thereby enhancing food safety and minimizing contamination. |
| format | Article |
| id | doaj-art-9125ec4b442b4eadbaa2c00146a75a89 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-04-01 |
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| series | Microorganisms |
| spelling | doaj-art-9125ec4b442b4eadbaa2c00146a75a892025-08-20T03:13:45ZengMDPI AGMicroorganisms2076-26072025-04-0113491410.3390/microorganisms13040914Effects of Fucoidan on the Inhibition of Biofilm Formation of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Serovar <i>Typhimurium</i> on Seafoods and Its Molecular Antibiofilm MechanismsAnamika Roy0Pantu Kumar Roy1Sung Rae Cho2Shin Young Park3Department of Seafood Science and Technology, Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Republic of KoreaDepartment of Seafood Science and Technology, Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Republic of KoreaSouth Sea Fisheries Research Institute, National Institute of Fisheries Science, Yeosu 59780, Republic of KoreaDepartment of Seafood Science and Technology, Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Republic of KoreaFoodborne illnesses, particularly those caused by <i>Salmonella enterica</i> subsp. <i>enterica</i> Serovar <i>Typhimurium</i>, present a significant challenge to public health, especially within the seafood industry due to biofilm formation on foods. This study investigated the antibiofilm potential of fucoidan, a sulfated polysaccharide, against <i>Salmonella enterica</i> subsp. <i>enterica</i> Serovar <i>Typhimurium</i> biofilm on crab and shrimp surfaces. Fucoidan’s minimum inhibitory concentration (MIC) was determined to be 150 µg/mL. Sub-MIC (1/8, 1/4, 1/2, and MIC) were evaluated for their impact on inhibition of biofilm formation. Fucoidan treatment resulted in significant, dose-dependent inhibition in biofilm formation, achieving 2.61 log CFU/cm<sup>2</sup> and 2.45 log CFU/cm<sup>2</sup> reductions on crab and shrimp surfaces, respectively. FE-SEM analysis confirmed biofilm disruption and cell membrane damage. Real-time PCR showed the downregulation of quorum-sensing (<i>luxS</i>) and virulence (<i>rpoS</i>, <i>avrA</i>, and <i>hilA</i>) genes. These results propose that fucoidan has the ability as a natural antibacterial agent for controlling <i>Salmonella enterica</i> subsp. <i>enterica</i> Serovar <i>Typhimurium</i> biofilms in seafood processing, thereby enhancing food safety and minimizing contamination.https://www.mdpi.com/2076-2607/13/4/914biofilms<i>Salmonella enterica</i> subsp. <i>enterica</i> Serovar <i>Typhimurium</i>crab shellsshrimp shellsfucoidan |
| spellingShingle | Anamika Roy Pantu Kumar Roy Sung Rae Cho Shin Young Park Effects of Fucoidan on the Inhibition of Biofilm Formation of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Serovar <i>Typhimurium</i> on Seafoods and Its Molecular Antibiofilm Mechanisms Microorganisms biofilms <i>Salmonella enterica</i> subsp. <i>enterica</i> Serovar <i>Typhimurium</i> crab shells shrimp shells fucoidan |
| title | Effects of Fucoidan on the Inhibition of Biofilm Formation of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Serovar <i>Typhimurium</i> on Seafoods and Its Molecular Antibiofilm Mechanisms |
| title_full | Effects of Fucoidan on the Inhibition of Biofilm Formation of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Serovar <i>Typhimurium</i> on Seafoods and Its Molecular Antibiofilm Mechanisms |
| title_fullStr | Effects of Fucoidan on the Inhibition of Biofilm Formation of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Serovar <i>Typhimurium</i> on Seafoods and Its Molecular Antibiofilm Mechanisms |
| title_full_unstemmed | Effects of Fucoidan on the Inhibition of Biofilm Formation of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Serovar <i>Typhimurium</i> on Seafoods and Its Molecular Antibiofilm Mechanisms |
| title_short | Effects of Fucoidan on the Inhibition of Biofilm Formation of <i>Salmonella enterica</i> Subsp. <i>enterica</i> Serovar <i>Typhimurium</i> on Seafoods and Its Molecular Antibiofilm Mechanisms |
| title_sort | effects of fucoidan on the inhibition of biofilm formation of i salmonella enterica i subsp i enterica i serovar i typhimurium i on seafoods and its molecular antibiofilm mechanisms |
| topic | biofilms <i>Salmonella enterica</i> subsp. <i>enterica</i> Serovar <i>Typhimurium</i> crab shells shrimp shells fucoidan |
| url | https://www.mdpi.com/2076-2607/13/4/914 |
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