Cloning a Chloroplast Genome in Saccharomyces cerevisiae and Escherichia coli
Chloroplast genomes present an alternative strategy for large-scale engineering of photosynthetic eukaryotes. Prior to our work, the chloroplast genomes of Chlamydomonas reinhardtii (204 kb) and Zea mays (140 kb) had been cloned using bacterial and yeast artificial chromosome (BAC/YAC) libraries, re...
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Main Authors: | Emma Walker, Bogumil Karas |
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Format: | Article |
Language: | English |
Published: |
Bio-protocol LLC
2025-01-01
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Series: | Bio-Protocol |
Online Access: | https://bio-protocol.org/en/bpdetail?id=5162&type=0 |
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