Cloning a Chloroplast Genome in Saccharomyces cerevisiae and Escherichia coli

Chloroplast genomes present an alternative strategy for large-scale engineering of photosynthetic eukaryotes. Prior to our work, the chloroplast genomes of Chlamydomonas reinhardtii (204 kb) and Zea mays (140 kb) had been cloned using bacterial and yeast artificial chromosome (BAC/YAC) libraries, re...

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Bibliographic Details
Main Authors: Emma Walker, Bogumil Karas
Format: Article
Language:English
Published: Bio-protocol LLC 2025-01-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5162&type=0
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