Topologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolutionResearch in context

Topologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolutionResearch in context

Summary: Background: The widespread and evolution of RNA viruses, such as the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), highlights the importance of fast identification of virus subtypes, particularly in non-laboratory settings. Rapid and inexpensive at-home testing of viral nuc...

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Main Authors: Yanan Li, Ru Xu, Fenglei Quan, Yonghua Wu, Yige Wu, Yongyuan Zhang, Yan Liang, Zhenzhong Zhang, Hua Gao, Ruijie Deng, Kaixiang Zhang, Jinghong Li
Format: Article
Language:English
Published: Elsevier 2025-02-01
Series:EBioMedicine
Subjects:
CRISPR diagnostic
Single base mutation
One-pot assay
Topologically constrained DNA rings
SARS-CoV-2 variants
Online Access:http://www.sciencedirect.com/science/article/pii/S2352396425000088
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http://www.sciencedirect.com/science/article/pii/S2352396425000088

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