CRISPR/Cas system-guided plasmid mutagenesis without sequence restriction

CRISPR/Cas system-guided plasmid mutagenesis without sequence restriction

Plasmid mutagenesis is an essential step to engineer protein variants with desired properties. Direct generation of mutations on plasmids is a promising method compared to PCR-based methods and subcloning, yet it suffers from the sequence restriction in the generation of single-stranded circular pla...

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Bibliographic Details
Main Authors: Fengjiao Zhao, Feng Chen, Huahang Yu, Siyue Fan, Min Bai, Jing Xue, Yue Zhao, Xiaolei Zuo, Chunhai Fan, Yongxi Zhao
Format: Article
Language:English
Published: KeAi Communications Co. Ltd. 2025-07-01
Series:Fundamental Research
Subjects:
DNA assembly
Plasmid mutation
CRISPR/Cas system
Sequence programmability
Protein engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2667325822002874
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http://www.sciencedirect.com/science/article/pii/S2667325822002874

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