BcACS6 overexpression promotes the accumulation of L-cysteine and L-tyrosine and improves the quality of Wucai
At present, the research on the quality of Wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen et Lee.) is still in its infancy. The content and type of amino acids play a pivotal role in determining the quality of Wucai. This study employed widely targeted metabolomics technology to ex...
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| Language: | English |
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Maximum Academic Press
2025-01-01
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| Series: | Vegetable Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/vegres-0025-0003 |
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| author | Wenying Zhang Guoliang Li Mengnan Xiong Qiuhong Chen Xun Gao Kangkang Wang Zhengyan Zhao Lixue Zhou Youzhi Liu Jinfeng Hou Wenjie Wang Jianqiang Wu Guohu Chen Xiaoyan Tang Lingyun Yuan Chenggang Wang |
| author_facet | Wenying Zhang Guoliang Li Mengnan Xiong Qiuhong Chen Xun Gao Kangkang Wang Zhengyan Zhao Lixue Zhou Youzhi Liu Jinfeng Hou Wenjie Wang Jianqiang Wu Guohu Chen Xiaoyan Tang Lingyun Yuan Chenggang Wang |
| author_sort | Wenying Zhang |
| collection | DOAJ |
| description | At present, the research on the quality of Wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen et Lee.) is still in its infancy. The content and type of amino acids play a pivotal role in determining the quality of Wucai. This study employed widely targeted metabolomics technology to examine the changes in metabolites associated with the quality transformation of Wucai, identifying amino acids as prominent differentially accumulated metabolites during this process. Electronic tongue analysis revealed that the primary taste attributes of Wucai before and after quality transformation are saltiness, astringency, and umami. To elucidate the relationship between amino acid composition and taste, we analyzed the correlation between electronic tongue response values and the reaction intensity data of flavor amino acids. The results showed that the absolute value of the correlation coefficient was highest between L-cysteine and umami, as well as between astringency and L-aspartic acid. Moreover, the values for L-phenylalanine, L-tyrosine, and γ-aminobutyric acid with saltiness were identical and surpassed those for all other amino acids. Transcriptomic analysis further revealed that BcACS6 was significantly positively correlated with changes in amino acid content, and its overexpression in Arabidopsis significantly increased the content of five amino acids associated with the primary taste attributes of Wucai, before and after its quality transformation. Additionally, BcCOR413PM1, BcBcDnaJ, and BcPCR8 were found to interact with BcACS6, suggesting that BcACS6 may be involved in protein synthesis and degradation during low-temperature acclimation, thereby regulating amino acid content and influencing flavor development in Wucai. |
| format | Article |
| id | doaj-art-8f60f28bb123438daa6f40bce66aee21 |
| institution | OA Journals |
| issn | 2769-0520 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Vegetable Research |
| spelling | doaj-art-8f60f28bb123438daa6f40bce66aee212025-08-20T02:27:15ZengMaximum Academic PressVegetable Research2769-05202025-01-015111110.48130/vegres-0025-0003vegres-0025-0003BcACS6 overexpression promotes the accumulation of L-cysteine and L-tyrosine and improves the quality of WucaiWenying Zhang0Guoliang Li1Mengnan Xiong2Qiuhong Chen3Xun Gao4Kangkang Wang5Zhengyan Zhao6Lixue Zhou7Youzhi Liu8Jinfeng Hou9Wenjie Wang10Jianqiang Wu11Guohu Chen12Xiaoyan Tang13Lingyun Yuan14Chenggang Wang15Vegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaVegetable Genetics and Breeding Laboratory, College of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaAt present, the research on the quality of Wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen et Lee.) is still in its infancy. The content and type of amino acids play a pivotal role in determining the quality of Wucai. This study employed widely targeted metabolomics technology to examine the changes in metabolites associated with the quality transformation of Wucai, identifying amino acids as prominent differentially accumulated metabolites during this process. Electronic tongue analysis revealed that the primary taste attributes of Wucai before and after quality transformation are saltiness, astringency, and umami. To elucidate the relationship between amino acid composition and taste, we analyzed the correlation between electronic tongue response values and the reaction intensity data of flavor amino acids. The results showed that the absolute value of the correlation coefficient was highest between L-cysteine and umami, as well as between astringency and L-aspartic acid. Moreover, the values for L-phenylalanine, L-tyrosine, and γ-aminobutyric acid with saltiness were identical and surpassed those for all other amino acids. Transcriptomic analysis further revealed that BcACS6 was significantly positively correlated with changes in amino acid content, and its overexpression in Arabidopsis significantly increased the content of five amino acids associated with the primary taste attributes of Wucai, before and after its quality transformation. Additionally, BcCOR413PM1, BcBcDnaJ, and BcPCR8 were found to interact with BcACS6, suggesting that BcACS6 may be involved in protein synthesis and degradation during low-temperature acclimation, thereby regulating amino acid content and influencing flavor development in Wucai.https://www.maxapress.com/article/doi/10.48130/vegres-0025-0003wucaiamino acidselectronic tonguefunctional verificationprotein interaction |
| spellingShingle | Wenying Zhang Guoliang Li Mengnan Xiong Qiuhong Chen Xun Gao Kangkang Wang Zhengyan Zhao Lixue Zhou Youzhi Liu Jinfeng Hou Wenjie Wang Jianqiang Wu Guohu Chen Xiaoyan Tang Lingyun Yuan Chenggang Wang BcACS6 overexpression promotes the accumulation of L-cysteine and L-tyrosine and improves the quality of Wucai Vegetable Research wucai amino acids electronic tongue functional verification protein interaction |
| title | BcACS6 overexpression promotes the accumulation of L-cysteine and L-tyrosine and improves the quality of Wucai |
| title_full | BcACS6 overexpression promotes the accumulation of L-cysteine and L-tyrosine and improves the quality of Wucai |
| title_fullStr | BcACS6 overexpression promotes the accumulation of L-cysteine and L-tyrosine and improves the quality of Wucai |
| title_full_unstemmed | BcACS6 overexpression promotes the accumulation of L-cysteine and L-tyrosine and improves the quality of Wucai |
| title_short | BcACS6 overexpression promotes the accumulation of L-cysteine and L-tyrosine and improves the quality of Wucai |
| title_sort | bcacs6 overexpression promotes the accumulation of l cysteine and l tyrosine and improves the quality of wucai |
| topic | wucai amino acids electronic tongue functional verification protein interaction |
| url | https://www.maxapress.com/article/doi/10.48130/vegres-0025-0003 |
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