Selection and validation of reference genes for gene expression studies in Pseudomonas brassicacearum GS20 using real-time quantitative reverse transcription PCR.

Selection and validation of reference genes for gene expression studies in Pseudomonas brassicacearum GS20 using real-time quantitative reverse transcription PCR.

Pseudomonas brassicacearum GS20 is an antagonistic strain of bacteria recently isolated from the rhizosphere of Codonopsis pilosula. No validated reference gene has been indentified from P. brassicacearum to use in the normalization of real-time quantitative reverse transcription-PCR data. Therefore...

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Bibliographic Details
Main Authors: Bianxia Bai, Jiahong Ren, Fenling Bai, Lin Hao
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0227927/1/pone.0227927.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210221%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210221T071523Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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