pTripleTREP – A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus

pTripleTREP – A vector for tightly controlled expression and purification of virulence factors in Staphylococcus aureus

Abstract Background Recombinant proteins facilitate and contribute to detailed studies of the virulence mechanisms and pathophysiology of the major human pathogen Staphylococcus aureus. Of particular interest are secreted virulence factors. However, due to their potential toxicity and specific post-...

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Main Authors: Hannes Wolfgramm, Christopher Saade, Marco Harms, Larissa M. Busch, Josephine Lange, Maximilian Schedlowski, Kristin Surmann, Manuela Gesell Salazar, Christian Hentschker, Leif Steil, Stephan Michalik, Uwe Völker, Alexander Reder
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Microbial Cell Factories
Subjects:
Homologous expression
Protein purification
Expression vector
Staphylococcus aureus
Twin-Strep-tag
Tetracycline-inducible promoter
Online Access:https://doi.org/10.1186/s12934-025-02736-7
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https://doi.org/10.1186/s12934-025-02736-7

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