RQdeltaCT: an open-source R package for relative quantification of gene expression using delta Ct methods

Abstract Relative quantification of gene expression is a fundamental method used in the molecular biology field to analyse real-time PCR data to determine transcriptional differences between groups of samples (or technical replicates of a single sample). The main methods used for relative quantifica...

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Bibliographic Details
Main Authors: Daniel Zalewski, Anna Bogucka-Kocka
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-025-11822-0
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