Efficient extracellular expression and antimicrobial activity of Procambarus clarkii invertebrate-type lysozyme in Pichia pastoris

Efficient extracellular expression and antimicrobial activity of Procambarus clarkii invertebrate-type lysozyme in Pichia pastoris

A Pichia pastoris expression method for the efficient extracellular production of Procambarus clarkii invertebrate-type lysozyme 1 (pc-iLys1) was established. First, the synthetic codon-optimized cDNA encoding pc-iLys1 was expressed in the P. pastoris SMD1168 with a molecular mass of about 17.1 kDa....

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Bibliographic Details
Main Authors: SHUI Yan, GUAN Zhengbing, YE Junxian, SHI Yonghong, LIU Guofeng, XU Zenghong
Format: Article
Language:English
Published: Zhejiang University Press 2019-10-01
Series:浙江大学学报. 农业与生命科学版
Subjects:
invertebrate-type lysozyme
<italic>Procambarus clarkii</italic>
<italic>Pichia pastoris</italic>
expression condition optimization
antimicrobial activity
Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.10.081
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https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.10.081

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