Protocol to isolate stress granules in HeLa cells using fluorescence-activated non-membrane condensate isolation

Summary: Various stress stimuli induce cytosolic stress granules (SGs) in mammalian cells, which are composed of RNA and RNA-binding proteins and have important physiological functions. Here, we present a protocol for isolating SGs using fluorescence-activated non-membrane condensate isolation (FANC...

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Bibliographic Details
Main Authors: Yilong Zhou, Maria Shvedunova, Asifa Akhtar
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003934
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