Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and <i>Nicotiana benthamiana</i>
Gene-editing technology, specifically the CRISPR-Cas13a system, has shown promise in breeding plants resistant to RNA viruses. This system targets RNA and, theoretically, can also combat RNA-based viroids. To test this, the CRISPR-Cas13a system was introduced into tomato plants via transient express...
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2024-08-01
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| Online Access: | https://www.mdpi.com/1999-4915/16/9/1401 |
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| author | Ying Wei Khoo Qingsong Wang Shangwu Liu Binhui Zhan Tengfei Xu Wenxia Lv Guangjing Liu Shifang Li Zhixiang Zhang |
| author_facet | Ying Wei Khoo Qingsong Wang Shangwu Liu Binhui Zhan Tengfei Xu Wenxia Lv Guangjing Liu Shifang Li Zhixiang Zhang |
| author_sort | Ying Wei Khoo |
| collection | DOAJ |
| description | Gene-editing technology, specifically the CRISPR-Cas13a system, has shown promise in breeding plants resistant to RNA viruses. This system targets RNA and, theoretically, can also combat RNA-based viroids. To test this, the CRISPR-Cas13a system was introduced into tomato plants via transient expression and into <i>Nicotiana benthamiana</i> through transgenic methods, using CRISPR RNAs (crRNAs) targeting the conserved regions of both sense and antisense genomes of potato spindle tuber viroid (PSTVd). In tomato plants, the expression of CRISPR-Cas13a and crRNAs substantially reduced PSTVd accumulation and alleviated disease symptoms. In transgenic <i>N. benthamiana</i> plants, the PSTVd levels were lower as compared to wild-type plants. Several effective crRNAs targeting the PSTVd genomic RNA were also identified. These results demonstrate that the CRISPR-Cas13a system can effectively target and combat viroid RNAs, despite their compact structures. |
| format | Article |
| id | doaj-art-8ce6578324cb44f4863152d28310f58a |
| institution | OA Journals |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-8ce6578324cb44f4863152d28310f58a2025-08-20T01:56:13ZengMDPI AGViruses1999-49152024-08-01169140110.3390/v16091401Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and <i>Nicotiana benthamiana</i>Ying Wei Khoo0Qingsong Wang1Shangwu Liu2Binhui Zhan3Tengfei Xu4Wenxia Lv5Guangjing Liu6Shifang Li7Zhixiang Zhang8State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaInstitute of Industrial Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaDepartment of Fruit Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaInner Mongolia Zhongjia Agricultural Biotechnology Co., Ltd., Ulanqab 011800, ChinaInner Mongolia Zhongjia Agricultural Biotechnology Co., Ltd., Ulanqab 011800, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaGene-editing technology, specifically the CRISPR-Cas13a system, has shown promise in breeding plants resistant to RNA viruses. This system targets RNA and, theoretically, can also combat RNA-based viroids. To test this, the CRISPR-Cas13a system was introduced into tomato plants via transient expression and into <i>Nicotiana benthamiana</i> through transgenic methods, using CRISPR RNAs (crRNAs) targeting the conserved regions of both sense and antisense genomes of potato spindle tuber viroid (PSTVd). In tomato plants, the expression of CRISPR-Cas13a and crRNAs substantially reduced PSTVd accumulation and alleviated disease symptoms. In transgenic <i>N. benthamiana</i> plants, the PSTVd levels were lower as compared to wild-type plants. Several effective crRNAs targeting the PSTVd genomic RNA were also identified. These results demonstrate that the CRISPR-Cas13a system can effectively target and combat viroid RNAs, despite their compact structures.https://www.mdpi.com/1999-4915/16/9/1401tomatoviroidgene editingCas13agenetic engineeringguide RNAs |
| spellingShingle | Ying Wei Khoo Qingsong Wang Shangwu Liu Binhui Zhan Tengfei Xu Wenxia Lv Guangjing Liu Shifang Li Zhixiang Zhang Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and <i>Nicotiana benthamiana</i> Viruses tomato viroid gene editing Cas13a genetic engineering guide RNAs |
| title | Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and <i>Nicotiana benthamiana</i> |
| title_full | Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and <i>Nicotiana benthamiana</i> |
| title_fullStr | Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and <i>Nicotiana benthamiana</i> |
| title_full_unstemmed | Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and <i>Nicotiana benthamiana</i> |
| title_short | Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and <i>Nicotiana benthamiana</i> |
| title_sort | resistance of the crispr cas13a gene editing system to potato spindle tuber viroid infection in tomato and i nicotiana benthamiana i |
| topic | tomato viroid gene editing Cas13a genetic engineering guide RNAs |
| url | https://www.mdpi.com/1999-4915/16/9/1401 |
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