Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and <i>Nicotiana benthamiana</i>

Resistance of the CRISPR-Cas13a Gene-Editing System to Potato Spindle Tuber Viroid Infection in Tomato and <i>Nicotiana benthamiana</i>

Gene-editing technology, specifically the CRISPR-Cas13a system, has shown promise in breeding plants resistant to RNA viruses. This system targets RNA and, theoretically, can also combat RNA-based viroids. To test this, the CRISPR-Cas13a system was introduced into tomato plants via transient express...

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Bibliographic Details
Main Authors: Ying Wei Khoo, Qingsong Wang, Shangwu Liu, Binhui Zhan, Tengfei Xu, Wenxia Lv, Guangjing Liu, Shifang Li, Zhixiang Zhang
Format: Article
Language:English
Published: MDPI AG 2024-08-01
Series:Viruses
Subjects:
tomato
viroid
gene editing
Cas13a
genetic engineering
guide RNAs
Online Access:https://www.mdpi.com/1999-4915/16/9/1401
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