Evolution of the PRNT: Merging tradition and innovation to set the gold standard in the era of automation
The plaque reduction neutralization test (PRNT) is the gold standard for quantifying neutralizing antibodies owing to its high sensitivity and specificity. Widely used in seroepidemiological studies and vaccine development, PRNT is limited by labor-intensive procedures, reliance on highly skilled st...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Human Vaccines & Immunotherapeutics |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21645515.2025.2528368 |
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| author | Caio Denani Ingrid Horbach Bruno Setatino Adriana Azevedo Sheila Lima Waleska Schwarcz Ivanildo Sousa |
| author_facet | Caio Denani Ingrid Horbach Bruno Setatino Adriana Azevedo Sheila Lima Waleska Schwarcz Ivanildo Sousa |
| author_sort | Caio Denani |
| collection | DOAJ |
| description | The plaque reduction neutralization test (PRNT) is the gold standard for quantifying neutralizing antibodies owing to its high sensitivity and specificity. Widely used in seroepidemiological studies and vaccine development, PRNT is limited by labor-intensive procedures, reliance on highly skilled staff, and susceptibility to human bias during manual plaque counting. These limitations hinder reproducibility, throughput, and cross-laboratory standardization. This review summarizes recent efforts to automate PRNT workflows to address these issues. However, automation adoption is often constrained by high costs and the need for regulatory frameworks to ensure data integrity. Advancements in tailored hardware and software to diverse laboratory contexts are essential to maintaining PRNT’s role in immunological assessments, bridging seroneutralizing methodologies with modern automation, and making automated methods more accessible. This review outlines the key requirements for PRNT’s continued evolution. |
| format | Article |
| id | doaj-art-8bd670cdaeab413baed8feee4b860576 |
| institution | Kabale University |
| issn | 2164-5515 2164-554X |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj-art-8bd670cdaeab413baed8feee4b8605762025-08-20T03:28:55ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2025-12-0121110.1080/21645515.2025.2528368Evolution of the PRNT: Merging tradition and innovation to set the gold standard in the era of automationCaio Denani0Ingrid Horbach1Bruno Setatino2Adriana Azevedo3Sheila Lima4Waleska Schwarcz5Ivanildo Sousa6Laboratório de Análise Imunomolecular, Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, BrasilLaboratório de Análise Imunomolecular, Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, BrasilLaboratório de Análise Imunomolecular, Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, BrasilLaboratório de Análise Imunomolecular, Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, BrasilDepartamento de Desenvolvimento Experimental e Pré-clínico (DEDEP), Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, BrasilLaboratório de Análise Imunomolecular, Instituto de Tecnologia em Imunobiológicos Bio-Manguinhos, Fundação Oswaldo Cruz, Rio de Janeiro, BrasilLaboratório de Virologia e Parasitologia Molecular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrasilThe plaque reduction neutralization test (PRNT) is the gold standard for quantifying neutralizing antibodies owing to its high sensitivity and specificity. Widely used in seroepidemiological studies and vaccine development, PRNT is limited by labor-intensive procedures, reliance on highly skilled staff, and susceptibility to human bias during manual plaque counting. These limitations hinder reproducibility, throughput, and cross-laboratory standardization. This review summarizes recent efforts to automate PRNT workflows to address these issues. However, automation adoption is often constrained by high costs and the need for regulatory frameworks to ensure data integrity. Advancements in tailored hardware and software to diverse laboratory contexts are essential to maintaining PRNT’s role in immunological assessments, bridging seroneutralizing methodologies with modern automation, and making automated methods more accessible. This review outlines the key requirements for PRNT’s continued evolution.https://www.tandfonline.com/doi/10.1080/21645515.2025.2528368PRNTautomationneutralizing antibodiesvaccine development |
| spellingShingle | Caio Denani Ingrid Horbach Bruno Setatino Adriana Azevedo Sheila Lima Waleska Schwarcz Ivanildo Sousa Evolution of the PRNT: Merging tradition and innovation to set the gold standard in the era of automation Human Vaccines & Immunotherapeutics PRNT automation neutralizing antibodies vaccine development |
| title | Evolution of the PRNT: Merging tradition and innovation to set the gold standard in the era of automation |
| title_full | Evolution of the PRNT: Merging tradition and innovation to set the gold standard in the era of automation |
| title_fullStr | Evolution of the PRNT: Merging tradition and innovation to set the gold standard in the era of automation |
| title_full_unstemmed | Evolution of the PRNT: Merging tradition and innovation to set the gold standard in the era of automation |
| title_short | Evolution of the PRNT: Merging tradition and innovation to set the gold standard in the era of automation |
| title_sort | evolution of the prnt merging tradition and innovation to set the gold standard in the era of automation |
| topic | PRNT automation neutralizing antibodies vaccine development |
| url | https://www.tandfonline.com/doi/10.1080/21645515.2025.2528368 |
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