A powerful and versatile new fixation protocol for immunostaining and in situ hybridization that preserves delicate tissues

A powerful and versatile new fixation protocol for immunostaining and in situ hybridization that preserves delicate tissues

Abstract Background Understanding how genes function to heal wounds and restore lost tissue is essential for studying regeneration. Whole-mount in situ hybridization (WISH) is a powerful and widely used technique to visualize the expression patterns of genes in different biological systems. Yet, exi...

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Bibliographic Details
Main Authors: Carlos Guerrero-Hernández, Viraj Doddihal, Frederick G. Mann, Alejandro Sánchez Alvarado
Format: Article
Language:English
Published: BMC 2024-11-01
Series:BMC Biology
Subjects:
WISH/FISH
In situ hybridization
Immunostaining
Regeneration
Wound healing
Blastema
Online Access:https://doi.org/10.1186/s12915-024-02052-3
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https://doi.org/10.1186/s12915-024-02052-3

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