Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction
Abstract Biochips are widely applied to manipulate the geometrical morphology of stem cells in recent years. Patterned antenna-like pseudopodia are also probed to explore the influence of pseudopodia formation on gene delivery and expression on biochips. However, how the antenna-like pseudopodia aff...
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BMC
2025-01-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03101-x |
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| author | Mingkui Shen Yan Hou Shihui Xu Jun Tan Honggang Zhou Qi Miao Wanheng Zhang Yazhou Chen Nana Wang Yongtao Wang |
| author_facet | Mingkui Shen Yan Hou Shihui Xu Jun Tan Honggang Zhou Qi Miao Wanheng Zhang Yazhou Chen Nana Wang Yongtao Wang |
| author_sort | Mingkui Shen |
| collection | DOAJ |
| description | Abstract Biochips are widely applied to manipulate the geometrical morphology of stem cells in recent years. Patterned antenna-like pseudopodia are also probed to explore the influence of pseudopodia formation on gene delivery and expression on biochips. However, how the antenna-like pseudopodia affect gene transfection is unsettled and the underlying trafficking mechanism of exogenous genes in engineered single cells is not announced. Therefore, the engineered microarray biochips were conceptualized and prepared by the synthesized photointelligent biopolymer to precisely manage geometric topological structures (cell size and antenna-like protrusion) of stem cells on biochips. The cytoskeleton could be regulated in engineered cells and large cells with more antennas assembled well-organized actin filaments to affect cell tension distribution. The stiffness and adhesion force were measured by atomic force microscope to reveal cell nanomechanics on microarray biochips. Cytoskeleton-mediated nanomechanics could be adjusted by actin filaments. Gene transfection efficiency was enhanced with increasing cell nanomechanics, which was also confirmed by the evaluation of cell internalization capacity of nanoparticles and DNA synthesis ability. This work will provide a new strategy to study functional biomaterials, microarray chips and internal mechanism of gene transfection in patterned stem cells on biochips. Graphical abstract |
| format | Article |
| id | doaj-art-8b9165a7b9a94f4dae9f08d1bed156a1 |
| institution | Kabale University |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-8b9165a7b9a94f4dae9f08d1bed156a12025-01-19T12:37:52ZengBMCJournal of Nanobiotechnology1477-31552025-01-0123111810.1186/s12951-025-03101-xBiofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransductionMingkui Shen0Yan Hou1Shihui Xu2Jun Tan3Honggang Zhou4Qi Miao5Wanheng Zhang6Yazhou Chen7Nana Wang8Yongtao Wang9Department of Mini-Invasive Spinal Surgery, The Third People’s Hospital of Henan ProvinceSchool of Medicine, Shanghai UniversitySchool of Medicine, Shanghai UniversityDepartment of Mini-Invasive Spinal Surgery, The Third People’s Hospital of Henan ProvinceDepartment of Mini-Invasive Spinal Surgery, The Third People’s Hospital of Henan ProvinceDepartment of Pharmacy, China Pharmaceutical UniversityDepartment of Pharmacy, China Pharmaceutical UniversityMedical 3D Printing Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou UniversityDepartment of Pediatrics, School of Medicine, Shanghai General Hospital, Shanghai Jiao Tong UniversitySchool of Medicine, Shanghai UniversityAbstract Biochips are widely applied to manipulate the geometrical morphology of stem cells in recent years. Patterned antenna-like pseudopodia are also probed to explore the influence of pseudopodia formation on gene delivery and expression on biochips. However, how the antenna-like pseudopodia affect gene transfection is unsettled and the underlying trafficking mechanism of exogenous genes in engineered single cells is not announced. Therefore, the engineered microarray biochips were conceptualized and prepared by the synthesized photointelligent biopolymer to precisely manage geometric topological structures (cell size and antenna-like protrusion) of stem cells on biochips. The cytoskeleton could be regulated in engineered cells and large cells with more antennas assembled well-organized actin filaments to affect cell tension distribution. The stiffness and adhesion force were measured by atomic force microscope to reveal cell nanomechanics on microarray biochips. Cytoskeleton-mediated nanomechanics could be adjusted by actin filaments. Gene transfection efficiency was enhanced with increasing cell nanomechanics, which was also confirmed by the evaluation of cell internalization capacity of nanoparticles and DNA synthesis ability. This work will provide a new strategy to study functional biomaterials, microarray chips and internal mechanism of gene transfection in patterned stem cells on biochips. Graphical abstracthttps://doi.org/10.1186/s12951-025-03101-xIntelligent microarray biochipsPatterned stem cellsCytoskeletal nanomechanicsGene delivery and expressionMechanotransduction |
| spellingShingle | Mingkui Shen Yan Hou Shihui Xu Jun Tan Honggang Zhou Qi Miao Wanheng Zhang Yazhou Chen Nana Wang Yongtao Wang Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction Journal of Nanobiotechnology Intelligent microarray biochips Patterned stem cells Cytoskeletal nanomechanics Gene delivery and expression Mechanotransduction |
| title | Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction |
| title_full | Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction |
| title_fullStr | Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction |
| title_full_unstemmed | Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction |
| title_short | Biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pDNA nanolipoplexes in stem cells via mechanotransduction |
| title_sort | biofunctionalized patterned platform as microarray biochip to supervise delivery and expression of pdna nanolipoplexes in stem cells via mechanotransduction |
| topic | Intelligent microarray biochips Patterned stem cells Cytoskeletal nanomechanics Gene delivery and expression Mechanotransduction |
| url | https://doi.org/10.1186/s12951-025-03101-x |
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