Verification of the Introgression of <i>Narenga porphyrocoma</i> Germplasm into <i>Saccharum officinarum</i> Using Molecular Markers and GISH Analysis
Sugarcane (<i>Saccharum</i> spp.), a critical crop for sugar and bioenergy production, faces challenges in genetic improvement due to limited genetic diversity from selective breeding. Expanding genetic resources through intergeneric hybridization, particularly with <i>Narenga porp...
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2025-01-01
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| Series: | Agronomy |
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| author | Gang Wang Wei Zhang Yuanxia Qin Qingdan Wu Qinggan Liang Jiantao Wu Shengren Sun Zhuqing Wang Yuxing An Jianqiang Wang Qinnan Wang Hailong Chang |
| author_facet | Gang Wang Wei Zhang Yuanxia Qin Qingdan Wu Qinggan Liang Jiantao Wu Shengren Sun Zhuqing Wang Yuxing An Jianqiang Wang Qinnan Wang Hailong Chang |
| author_sort | Gang Wang |
| collection | DOAJ |
| description | Sugarcane (<i>Saccharum</i> spp.), a critical crop for sugar and bioenergy production, faces challenges in genetic improvement due to limited genetic diversity from selective breeding. Expanding genetic resources through intergeneric hybridization, particularly with <i>Narenga porphyrocoma</i>, offers a promising avenue to introduce traits like stress resistance and high biomass productivity. However, verifying true hybrids remains challenging with traditional morphological methods. This study employed tetra-primer ARMS-PCR and genomic in situ hybridization (GISH) to accurately identify intergeneric hybrids between <i>S. officinarum</i> and <i>N. porphyrocoma</i>. Species-specific primers were designed based on SNPs in the nrDNA-ITS region for ARMS-PCR, enabling effective differentiation of parental and hybrid genotypes, while GISH confirmed the chromosomal composition of hybrids, revealing an n + n inheritance pattern. The results demonstrated the potential of <i>N. porphyrocoma</i> to improve sugarcane’s tillering and leaf length, although sucrose content was lower in hybrids, suggesting the need for further breeding efforts. This study uniquely contributes to sugarcane breeding by providing an effective method for hybrid verification and laying a foundation for incorporating beneficial <i>N. porphyrocoma</i> genes into sugarcane cultivars. |
| format | Article |
| id | doaj-art-8b6a966408714d75a123b6c5df0da5d6 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-8b6a966408714d75a123b6c5df0da5d62025-01-24T13:16:48ZengMDPI AGAgronomy2073-43952025-01-0115112110.3390/agronomy15010121Verification of the Introgression of <i>Narenga porphyrocoma</i> Germplasm into <i>Saccharum officinarum</i> Using Molecular Markers and GISH AnalysisGang Wang0Wei Zhang1Yuanxia Qin2Qingdan Wu3Qinggan Liang4Jiantao Wu5Shengren Sun6Zhuqing Wang7Yuxing An8Jianqiang Wang9Qinnan Wang10Hailong Chang11Institute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaInstitute of Nanfan and Seed Industry, Guangdong Academy of Sciences, Guangzhou 510316, ChinaSugarcane (<i>Saccharum</i> spp.), a critical crop for sugar and bioenergy production, faces challenges in genetic improvement due to limited genetic diversity from selective breeding. Expanding genetic resources through intergeneric hybridization, particularly with <i>Narenga porphyrocoma</i>, offers a promising avenue to introduce traits like stress resistance and high biomass productivity. However, verifying true hybrids remains challenging with traditional morphological methods. This study employed tetra-primer ARMS-PCR and genomic in situ hybridization (GISH) to accurately identify intergeneric hybrids between <i>S. officinarum</i> and <i>N. porphyrocoma</i>. Species-specific primers were designed based on SNPs in the nrDNA-ITS region for ARMS-PCR, enabling effective differentiation of parental and hybrid genotypes, while GISH confirmed the chromosomal composition of hybrids, revealing an n + n inheritance pattern. The results demonstrated the potential of <i>N. porphyrocoma</i> to improve sugarcane’s tillering and leaf length, although sucrose content was lower in hybrids, suggesting the need for further breeding efforts. This study uniquely contributes to sugarcane breeding by providing an effective method for hybrid verification and laying a foundation for incorporating beneficial <i>N. porphyrocoma</i> genes into sugarcane cultivars.https://www.mdpi.com/2073-4395/15/1/121<i>Narenga porphyrocoma</i>intergeneric hybridsnrDNA-ITStetra-primer ARMS-PCRGISHgermplasm innovation |
| spellingShingle | Gang Wang Wei Zhang Yuanxia Qin Qingdan Wu Qinggan Liang Jiantao Wu Shengren Sun Zhuqing Wang Yuxing An Jianqiang Wang Qinnan Wang Hailong Chang Verification of the Introgression of <i>Narenga porphyrocoma</i> Germplasm into <i>Saccharum officinarum</i> Using Molecular Markers and GISH Analysis Agronomy <i>Narenga porphyrocoma</i> intergeneric hybrids nrDNA-ITS tetra-primer ARMS-PCR GISH germplasm innovation |
| title | Verification of the Introgression of <i>Narenga porphyrocoma</i> Germplasm into <i>Saccharum officinarum</i> Using Molecular Markers and GISH Analysis |
| title_full | Verification of the Introgression of <i>Narenga porphyrocoma</i> Germplasm into <i>Saccharum officinarum</i> Using Molecular Markers and GISH Analysis |
| title_fullStr | Verification of the Introgression of <i>Narenga porphyrocoma</i> Germplasm into <i>Saccharum officinarum</i> Using Molecular Markers and GISH Analysis |
| title_full_unstemmed | Verification of the Introgression of <i>Narenga porphyrocoma</i> Germplasm into <i>Saccharum officinarum</i> Using Molecular Markers and GISH Analysis |
| title_short | Verification of the Introgression of <i>Narenga porphyrocoma</i> Germplasm into <i>Saccharum officinarum</i> Using Molecular Markers and GISH Analysis |
| title_sort | verification of the introgression of i narenga porphyrocoma i germplasm into i saccharum officinarum i using molecular markers and gish analysis |
| topic | <i>Narenga porphyrocoma</i> intergeneric hybrids nrDNA-ITS tetra-primer ARMS-PCR GISH germplasm innovation |
| url | https://www.mdpi.com/2073-4395/15/1/121 |
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