Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure.

Formamide denaturation of double-stranded DNA for fluorescence in situ hybridization (FISH) distorts nanoscale chromatin structure.

As imaging techniques rapidly evolve to probe nanoscale genome organization at higher resolution, it is critical to consider how the reagents and procedures involved in sample preparation affect chromatin at the relevant length scales. Here, we investigate the effects of fluorescent labeling of DNA...

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Main Authors: Anne R Shim, Jane Frederick, Emily M Pujadas, Tiffany Kuo, I Chae Ye, Joshua A Pritchard, Cody L Dunton, Paola Carrillo Gonzalez, Nicolas Acosta, Surbhi Jain, Nicholas M Anthony, Luay M Almassalha, Igal Szleifer, Vadim Backman
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2024-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0301000
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https://doi.org/10.1371/journal.pone.0301000

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