Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7
This paper investigates differences in efficacy of isolating pathogenic bacteria Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7 between conventional cultivation (ISO method) and immunomagnetic separation (IMS method) related to the types of dairy products and initial num...
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| Language: | English |
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Croatian Dairy Union
2018-01-01
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| Series: | Mljekarstvo |
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| Online Access: | https://hrcak.srce.hr/file/283661 |
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| author | Boris Antunović Ivančica Kovaček Kristina Gvozdanović Manuela Grčević Vesna Gantner Vedran Poljak Mario Ostović Željko Pavičić Nihada Ahmetović |
| author_facet | Boris Antunović Ivančica Kovaček Kristina Gvozdanović Manuela Grčević Vesna Gantner Vedran Poljak Mario Ostović Željko Pavičić Nihada Ahmetović |
| author_sort | Boris Antunović |
| collection | DOAJ |
| description | This paper investigates differences in efficacy of isolating pathogenic bacteria Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7 between conventional cultivation (ISO method) and immunomagnetic separation (IMS method) related to the types of dairy products and initial numbers of bacteria. Different milk products (dairy pudding- vanilla or chocolate; a mixture of yoghurt and pudding; solid, liquid and fruit yoghurt; AB culture - with or without fruit; cheese spread) were intentionally contaminated with different numbers (≈10 and ≈30) of live cells of the observed bacteria per mL. The obtained results showed that the classical ISO procedure still represents an equally adequate method for the detection of S. Enteritidis and L. monocytogenes in dairy products as well as the IMS method. However, the ISO method was found to be inefficient for determination of E. coli O157:H7 when the initial contamination was low (≈10 live cells per mL). In such cases, even the IMS method appeared to be inefficient when used for fermented dairy product analysis. Fermented dairy products in contrast to the non-fermented ones, still represent a challenge for the development of routine detection methods, especially for S. Enteritidis, whilst the detection of L. monocytogenes and E. coli O157:H7 has improved by introducing the IMS method. The largest difference in the ability to detect bacteria in dairy product samples with reference to the initial number of bacteria by both methods was in the detection of E. coli O157:H7. The choice of broth (non-selective fluid broth vs. selective fluid broth) did not matter in the in the detection of S. Enteritidis and L. monocytogenes by applying the IMS procedure. However, for the detection of E. coli O157:H7 the application of modified tripton-soya broth with novobiocin (mTSB+Nb) has proved to be superior when compared to using the buffered peptone water (BPW). The presented results may be of importance as a scientific basis for future determination of standard methods related to laboratory detection of pathogens in dairy products. |
| format | Article |
| id | doaj-art-895ee240818b4b558169daa198b3404c |
| institution | DOAJ |
| issn | 0026-704X 1846-4025 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Croatian Dairy Union |
| record_format | Article |
| series | Mljekarstvo |
| spelling | doaj-art-895ee240818b4b558169daa198b3404c2025-08-20T03:07:16ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252018-01-0168131110.15567/mljekarstvo.2018.0101Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7Boris Antunović0Ivančica Kovaček1Kristina Gvozdanović2Manuela Grčević3Vesna Gantner4Vedran Poljak5Mario Ostović6Željko Pavičić7Nihada Ahmetović8University of J.J. Strossmayer, Faculty of Agriculture, Department for Animal Husbandry, Vladimira Preloga 1, 31000 Osijek, CroatiaDr. Andrija Štampar Institute of Public Health, Department of Environmental Protection and Health Ecology, Division of Food and Consumer Goods Microbiology, Mirogojska cesta 16, 10000 Zagreb, CroatiaUniversity of J.J. Strossmayer, Faculty of Agriculture, Department for Animal Husbandry, Vladimira Preloga 1, 31000 Osijek, CroatiaUniversity of J.J. Strossmayer, Faculty of Agriculture, Department for Animal Husbandry, Vladimira Preloga 1, 31000 Osijek, CroatiaUniversity of J.J. Strossmayer, Faculty of Agriculture, Department for Animal Husbandry, Vladimira Preloga 1, 31000 Osijek, CroatiaCroatian Institute of Public Health, Department of Health Ecology, Rockefellerova 7, 10000 Zagreb, CroatiaUniversity of Zagreb, Faculty of Veterinary Medicine, Department of Animal Hygiene, Behaviour and Welfare, Heinzelova 55, 10000 Zagreb, CroatiaUniversity of Zagreb, Faculty of Veterinary Medicine, Department of Animal Hygiene, Behaviour and Welfare, Heinzelova 55, 10000 Zagreb, CroatiaUniversity of Tuzla, Faculty of Medicine, Univerzitetska 1, Tuzla 75000, Bosnia and HerzegovinaThis paper investigates differences in efficacy of isolating pathogenic bacteria Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7 between conventional cultivation (ISO method) and immunomagnetic separation (IMS method) related to the types of dairy products and initial numbers of bacteria. Different milk products (dairy pudding- vanilla or chocolate; a mixture of yoghurt and pudding; solid, liquid and fruit yoghurt; AB culture - with or without fruit; cheese spread) were intentionally contaminated with different numbers (≈10 and ≈30) of live cells of the observed bacteria per mL. The obtained results showed that the classical ISO procedure still represents an equally adequate method for the detection of S. Enteritidis and L. monocytogenes in dairy products as well as the IMS method. However, the ISO method was found to be inefficient for determination of E. coli O157:H7 when the initial contamination was low (≈10 live cells per mL). In such cases, even the IMS method appeared to be inefficient when used for fermented dairy product analysis. Fermented dairy products in contrast to the non-fermented ones, still represent a challenge for the development of routine detection methods, especially for S. Enteritidis, whilst the detection of L. monocytogenes and E. coli O157:H7 has improved by introducing the IMS method. The largest difference in the ability to detect bacteria in dairy product samples with reference to the initial number of bacteria by both methods was in the detection of E. coli O157:H7. The choice of broth (non-selective fluid broth vs. selective fluid broth) did not matter in the in the detection of S. Enteritidis and L. monocytogenes by applying the IMS procedure. However, for the detection of E. coli O157:H7 the application of modified tripton-soya broth with novobiocin (mTSB+Nb) has proved to be superior when compared to using the buffered peptone water (BPW). The presented results may be of importance as a scientific basis for future determination of standard methods related to laboratory detection of pathogens in dairy products.https://hrcak.srce.hr/file/283661milkdairy productsSalmonella EnteritidisListeria monocytogenesEscherichia coli O157:H7immunomagnetic separation |
| spellingShingle | Boris Antunović Ivančica Kovaček Kristina Gvozdanović Manuela Grčević Vesna Gantner Vedran Poljak Mario Ostović Željko Pavičić Nihada Ahmetović Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7 Mljekarstvo milk dairy products Salmonella Enteritidis Listeria monocytogenes Escherichia coli O157:H7 immunomagnetic separation |
| title | Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7 |
| title_full | Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7 |
| title_fullStr | Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7 |
| title_full_unstemmed | Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7 |
| title_short | Influence of milk product type and its initial contamination on the efficiency of different methods for detection of Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7 |
| title_sort | influence of milk product type and its initial contamination on the efficiency of different methods for detection of salmonella enteritidis listeria monocytogenes and escherichia coli o157 h7 |
| topic | milk dairy products Salmonella Enteritidis Listeria monocytogenes Escherichia coli O157:H7 immunomagnetic separation |
| url | https://hrcak.srce.hr/file/283661 |
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