Antioxidative effects of α-tocopherol on stored human red blood cell units
BACKGROUND: Red blood cell (RBC) units undergo metabolic, structural, and biochemical changes known as “storage lesions” that can reduce the survival and quality of RBCs. The use of antioxidants such as α-tocopherol may help to improve the quality of RBC units by reducing oxidative stress. The aim o...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-01-01
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| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/ajts.ajts_130_22 |
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| author | Saeideh Hajizamani Kamran Atarodi Mohammad Reza Deyhim Fahimeh Ranjbar Kermani Kamran Mousavi Hosseini |
| author_facet | Saeideh Hajizamani Kamran Atarodi Mohammad Reza Deyhim Fahimeh Ranjbar Kermani Kamran Mousavi Hosseini |
| author_sort | Saeideh Hajizamani |
| collection | DOAJ |
| description | BACKGROUND:
Red blood cell (RBC) units undergo metabolic, structural, and biochemical changes known as “storage lesions” that can reduce the survival and quality of RBCs. The use of antioxidants such as α-tocopherol may help to improve the quality of RBC units by reducing oxidative stress. The aim of this study was to determine the antioxidant effect of α-tocopherol in RBC units containing citrate-phosphate-dextrose solution with adenine (CPDA1) stored at 1°C–6°C for 35 days.
MATERIALS AND METHODS:
Four RBC units containing CPDA1 were divided into four equal satellite bags. Three bags were supplemented with 0.125, 0.625, and 3.125 mM concentrations of α-tocopherol as test groups. One bag was supplemented with ethanol (0.5%) as a control group. They were stored at 1°C–6°C for 35 days. Malondialdehyde (MDA) concentration, total antioxidant capacity (TAC), and hemolysis index (HI) were measured on days 0, 7, 14, 21, 28, and 35.
RESULTS:
In all groups, MDA concentration and HI increased and TAC decreased (P < 0.05). MDA concentration and HI in the 3.125 mM of the α-tocopherol group had a lower increase compared to the other test and control groups. Supplementation of RBC units with α-tocopherol resulted in a significant increase of TAC in all three groups compared to the control group (P < 0.05) and had a lower reduction during storage.
CONCLUSION:
Supplementation of RBC units with α-tocopherol improves the quality of RBC units by decreasing lipid peroxidation and hemolysis and by increasing TAC. Among the mentioned concentrations, 3.125 mM of α-tocopherol had a significantly more antioxidant effect. |
| format | Article |
| id | doaj-art-8953a300a33949bcaa1e8931ff5bddd6 |
| institution | Kabale University |
| issn | 0973-6247 1998-3565 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj-art-8953a300a33949bcaa1e8931ff5bddd62025-01-20T09:39:46ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652024-01-0118110210710.4103/ajts.ajts_130_22Antioxidative effects of α-tocopherol on stored human red blood cell unitsSaeideh HajizamaniKamran AtarodiMohammad Reza DeyhimFahimeh Ranjbar KermaniKamran Mousavi HosseiniBACKGROUND: Red blood cell (RBC) units undergo metabolic, structural, and biochemical changes known as “storage lesions” that can reduce the survival and quality of RBCs. The use of antioxidants such as α-tocopherol may help to improve the quality of RBC units by reducing oxidative stress. The aim of this study was to determine the antioxidant effect of α-tocopherol in RBC units containing citrate-phosphate-dextrose solution with adenine (CPDA1) stored at 1°C–6°C for 35 days. MATERIALS AND METHODS: Four RBC units containing CPDA1 were divided into four equal satellite bags. Three bags were supplemented with 0.125, 0.625, and 3.125 mM concentrations of α-tocopherol as test groups. One bag was supplemented with ethanol (0.5%) as a control group. They were stored at 1°C–6°C for 35 days. Malondialdehyde (MDA) concentration, total antioxidant capacity (TAC), and hemolysis index (HI) were measured on days 0, 7, 14, 21, 28, and 35. RESULTS: In all groups, MDA concentration and HI increased and TAC decreased (P < 0.05). MDA concentration and HI in the 3.125 mM of the α-tocopherol group had a lower increase compared to the other test and control groups. Supplementation of RBC units with α-tocopherol resulted in a significant increase of TAC in all three groups compared to the control group (P < 0.05) and had a lower reduction during storage. CONCLUSION: Supplementation of RBC units with α-tocopherol improves the quality of RBC units by decreasing lipid peroxidation and hemolysis and by increasing TAC. Among the mentioned concentrations, 3.125 mM of α-tocopherol had a significantly more antioxidant effect.https://journals.lww.com/10.4103/ajts.ajts_130_22hemolysis indexmalondialdehydered blood cell unitstorage lesiontotal antioxidant capacityα-tocopherol |
| spellingShingle | Saeideh Hajizamani Kamran Atarodi Mohammad Reza Deyhim Fahimeh Ranjbar Kermani Kamran Mousavi Hosseini Antioxidative effects of α-tocopherol on stored human red blood cell units Asian Journal of Transfusion Science hemolysis index malondialdehyde red blood cell unit storage lesion total antioxidant capacity α-tocopherol |
| title | Antioxidative effects of α-tocopherol on stored human red blood cell units |
| title_full | Antioxidative effects of α-tocopherol on stored human red blood cell units |
| title_fullStr | Antioxidative effects of α-tocopherol on stored human red blood cell units |
| title_full_unstemmed | Antioxidative effects of α-tocopherol on stored human red blood cell units |
| title_short | Antioxidative effects of α-tocopherol on stored human red blood cell units |
| title_sort | antioxidative effects of α tocopherol on stored human red blood cell units |
| topic | hemolysis index malondialdehyde red blood cell unit storage lesion total antioxidant capacity α-tocopherol |
| url | https://journals.lww.com/10.4103/ajts.ajts_130_22 |
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