Evaluation of the killing effects of UV254 light on common airborne porcine viruses
UV exposure is a common method of disinfection and sterilization. In the present study, the parallel beam test was performed to collect fluids containing infectious viruses using a parallel beam apparatus after UV254 irradiation (0, 0.5, 1, 3, 5, 7, 10, or 20 mJ/cm2). The air sterilization test was...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1512387/full |
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| author | YingWu Qiu YingWu Qiu QunHui Li WenKai Zhao Hao Chang Hao Chang JunHua Wang Qi Gao Qi Gao Qingfeng Zhou GuiHong Zhang GuiHong Zhang Lang Gong Lang Gong LianXiang Wang |
| author_facet | YingWu Qiu YingWu Qiu QunHui Li WenKai Zhao Hao Chang Hao Chang JunHua Wang Qi Gao Qi Gao Qingfeng Zhou GuiHong Zhang GuiHong Zhang Lang Gong Lang Gong LianXiang Wang |
| author_sort | YingWu Qiu |
| collection | DOAJ |
| description | UV exposure is a common method of disinfection and sterilization. In the present study, the parallel beam test was performed to collect fluids containing infectious viruses using a parallel beam apparatus after UV254 irradiation (0, 0.5, 1, 3, 5, 7, 10, or 20 mJ/cm2). The air sterilization test was performed by irradiating the air in the ducts with UV254 light (0, 1, 2, 3, 4, or 6 mJ/cm2) to collect airborne particles containing viruses through the air sterilization equipment. Furthermore, viral inactivation was assessed based on cytopathic effect (CPE) detection and immunofluorescent assays (IFA). Both the CPE and immunofluorescence signal intensity decreased as the UV254 dose increased. The UV254 doses required to inactivate ASFV (107.75 copies/mL), PRRSV (106.29 copies/mL), and PEDV (107.71 copies/mL) in the water were 3, 1, and 1 mJ/cm2, respectively. The UV254 dose required to inactivate ASFV (104.06 copies/mL), PRRSV (103.06 copies/mL), and PEDV (104.68 copies/mL) in the air was 1 mJ/cm2. This study provides data required for biosecurity prevention and control in swine farms. |
| format | Article |
| id | doaj-art-892743d7b51c41c690c11b4582122c3f |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-892743d7b51c41c690c11b4582122c3f2025-01-31T12:01:17ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011210.3389/fvets.2025.15123871512387Evaluation of the killing effects of UV254 light on common airborne porcine virusesYingWu Qiu0YingWu Qiu1QunHui Li2WenKai Zhao3Hao Chang4Hao Chang5JunHua Wang6Qi Gao7Qi Gao8Qingfeng Zhou9GuiHong Zhang10GuiHong Zhang11Lang Gong12Lang Gong13LianXiang Wang14Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Livestock and Poultry Health and Environmental Control, Yunfu, ChinaGuangdong Provincial Key Laboratory of Livestock and Poultry Health and Environmental Control, Yunfu, ChinaGuangdong Provincial Key Laboratory of Livestock and Poultry Health and Environmental Control, Yunfu, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Livestock and Poultry Health and Environmental Control, Yunfu, ChinaFoshan Comwin Light & Electricity Co., Ltd., Foshan, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaAfrican Swine Fever Regional Laboratory of China (Guangzhou), Guangzhou, ChinaGuangdong Provincial Key Laboratory of Livestock and Poultry Health and Environmental Control, Yunfu, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaAfrican Swine Fever Regional Laboratory of China (Guangzhou), Guangzhou, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaAfrican Swine Fever Regional Laboratory of China (Guangzhou), Guangzhou, ChinaGuangdong Provincial Key Laboratory of Livestock and Poultry Health and Environmental Control, Yunfu, ChinaUV exposure is a common method of disinfection and sterilization. In the present study, the parallel beam test was performed to collect fluids containing infectious viruses using a parallel beam apparatus after UV254 irradiation (0, 0.5, 1, 3, 5, 7, 10, or 20 mJ/cm2). The air sterilization test was performed by irradiating the air in the ducts with UV254 light (0, 1, 2, 3, 4, or 6 mJ/cm2) to collect airborne particles containing viruses through the air sterilization equipment. Furthermore, viral inactivation was assessed based on cytopathic effect (CPE) detection and immunofluorescent assays (IFA). Both the CPE and immunofluorescence signal intensity decreased as the UV254 dose increased. The UV254 doses required to inactivate ASFV (107.75 copies/mL), PRRSV (106.29 copies/mL), and PEDV (107.71 copies/mL) in the water were 3, 1, and 1 mJ/cm2, respectively. The UV254 dose required to inactivate ASFV (104.06 copies/mL), PRRSV (103.06 copies/mL), and PEDV (104.68 copies/mL) in the air was 1 mJ/cm2. This study provides data required for biosecurity prevention and control in swine farms.https://www.frontiersin.org/articles/10.3389/fvets.2025.1512387/fullUV radiationair disinfectionASFVPRRSVPEDV |
| spellingShingle | YingWu Qiu YingWu Qiu QunHui Li WenKai Zhao Hao Chang Hao Chang JunHua Wang Qi Gao Qi Gao Qingfeng Zhou GuiHong Zhang GuiHong Zhang Lang Gong Lang Gong LianXiang Wang Evaluation of the killing effects of UV254 light on common airborne porcine viruses Frontiers in Veterinary Science UV radiation air disinfection ASFV PRRSV PEDV |
| title | Evaluation of the killing effects of UV254 light on common airborne porcine viruses |
| title_full | Evaluation of the killing effects of UV254 light on common airborne porcine viruses |
| title_fullStr | Evaluation of the killing effects of UV254 light on common airborne porcine viruses |
| title_full_unstemmed | Evaluation of the killing effects of UV254 light on common airborne porcine viruses |
| title_short | Evaluation of the killing effects of UV254 light on common airborne porcine viruses |
| title_sort | evaluation of the killing effects of uv254 light on common airborne porcine viruses |
| topic | UV radiation air disinfection ASFV PRRSV PEDV |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1512387/full |
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