Applications of mutagenesis methods on affinity maturation of antibodies in vitro

Applications of mutagenesis methods on affinity maturation of antibodies in vitro

Antibodies selected from naive antibody libraries usually have low affinity at the micromolar level. Meanwhile, the affinities of natural antibodies obtained by repeated immunization were also observed to have a limit around 100 picomole because of the inability to discriminate slower dissociation k...

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Bibliographic Details
Main Authors: LIU Yuan, LIN Manman, ZHANG Xiao, XU Chongxin, JIAO Linxia, ZHONG Jianfeng, WU Aihua, LIU Xianjin
Format: Article
Language:English
Published: Zhejiang University Press 2016-01-01
Series:浙江大学学报. 农业与生命科学版
Subjects:
antibodies
affinity maturation
error PCR
DNA shuffling
mutator strains
site-directed mutagenesis
chain shuffling
Online Access:https://www.academax.com/doi/10.3785/j.issn.1008-9209.2015.03.251
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https://www.academax.com/doi/10.3785/j.issn.1008-9209.2015.03.251

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