Frequency Of Bacterial Co-Infections Isolated from Covid-19 Positive Patients From Tertiary Care Hospital Of Karachi
Objective: This study aims to determine the frequency of bacterial co-infections in COVID-19-positive patients. Methodology: A prospective cross-sectional study was conducted in the Department of Microbiology, Pakistan, from November 15, 2021, to April 15, 2022. Blood and respiratory tract sampl...
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Rawalpindi Medical University
2023-06-01
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| Series: | Journal of Rawalpindi Medical College |
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| Online Access: | https://www.journalrmc.com/index.php/JRMC/article/view/2024 |
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| author | Fouzia Zeeshan Khan Ambreen Fatima Hareem Gohar Mehwish Sajjad Mona Siddiqui Saima Naseem |
| author_facet | Fouzia Zeeshan Khan Ambreen Fatima Hareem Gohar Mehwish Sajjad Mona Siddiqui Saima Naseem |
| author_sort | Fouzia Zeeshan Khan |
| collection | DOAJ |
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Objective: This study aims to determine the frequency of bacterial co-infections in COVID-19-positive patients.
Methodology: A prospective cross-sectional study was conducted in the Department of Microbiology, Pakistan, from November 15, 2021, to April 15, 2022. Blood and respiratory tract samples were collected, including sputum, bronchial lavage, and tracheal aspirate. Clinical specimens were inoculated onto a Sheep blood agar plate, Chocolate agar plate (aerobic with 5% CO2), and MacConkey’s agar. Identification was followed by specific and standard microbiological protocols. COVID-19 was confirmed by qualitative PCR. Antimicrobial susceptibility testing was performed using the Kirby Bauer disc diffusion method.
Results: A total of 202 clinical samples, including blood, sputum, tracheal aspirates, and bronchial lavage, were collected from COVID-19-positive patients. Male patients were more common in sputum and tracheal aspirates, while female patients were more common in blood cultures. The majority of patients were over 60 years of age. Acinetobacter baumannii was predominantly isolated from blood and tracheal aspirates, exhibiting multidrug resistance, but showing complete sensitivity towards Colistin. Klebsiella pneumonia exhibited high prevalence in sputum, with complete resistance observed in Cephalosporins and Co-trimoxazole.
Conclusion: The study concludes a high frequency of superadded bacterial co-infections, caused most prominently by Acinetobacter baumannii, Klebsiella pneumonia, and Pseudomonas aeruginosa. The majority of these are multidrug-resistant pathogens, therefore, urgent action is required to control the spread of nosocomial infections by resistant strains, which are responsible for the high mortality rate among COVID-19 critical patients.
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| format | Article |
| id | doaj-art-8803398b3d884f8ba72c066cf9cff9d7 |
| institution | Kabale University |
| issn | 1683-3562 1683-3570 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | Rawalpindi Medical University |
| record_format | Article |
| series | Journal of Rawalpindi Medical College |
| spelling | doaj-art-8803398b3d884f8ba72c066cf9cff9d72025-02-06T08:41:53ZengRawalpindi Medical UniversityJournal of Rawalpindi Medical College1683-35621683-35702023-06-0127210.37939/jrmc.v27i2.2024Frequency Of Bacterial Co-Infections Isolated from Covid-19 Positive Patients From Tertiary Care Hospital Of Karachi Fouzia Zeeshan KhanAmbreen FatimaHareem GoharMehwish SajjadMona SiddiquiSaima Naseem Objective: This study aims to determine the frequency of bacterial co-infections in COVID-19-positive patients. Methodology: A prospective cross-sectional study was conducted in the Department of Microbiology, Pakistan, from November 15, 2021, to April 15, 2022. Blood and respiratory tract samples were collected, including sputum, bronchial lavage, and tracheal aspirate. Clinical specimens were inoculated onto a Sheep blood agar plate, Chocolate agar plate (aerobic with 5% CO2), and MacConkey’s agar. Identification was followed by specific and standard microbiological protocols. COVID-19 was confirmed by qualitative PCR. Antimicrobial susceptibility testing was performed using the Kirby Bauer disc diffusion method. Results: A total of 202 clinical samples, including blood, sputum, tracheal aspirates, and bronchial lavage, were collected from COVID-19-positive patients. Male patients were more common in sputum and tracheal aspirates, while female patients were more common in blood cultures. The majority of patients were over 60 years of age. Acinetobacter baumannii was predominantly isolated from blood and tracheal aspirates, exhibiting multidrug resistance, but showing complete sensitivity towards Colistin. Klebsiella pneumonia exhibited high prevalence in sputum, with complete resistance observed in Cephalosporins and Co-trimoxazole. Conclusion: The study concludes a high frequency of superadded bacterial co-infections, caused most prominently by Acinetobacter baumannii, Klebsiella pneumonia, and Pseudomonas aeruginosa. The majority of these are multidrug-resistant pathogens, therefore, urgent action is required to control the spread of nosocomial infections by resistant strains, which are responsible for the high mortality rate among COVID-19 critical patients. https://www.journalrmc.com/index.php/JRMC/article/view/2024COVID-19, bacterial coinfection, Pseudomonas aeruginosa, Acinetobacter baumannii, MRSA |
| spellingShingle | Fouzia Zeeshan Khan Ambreen Fatima Hareem Gohar Mehwish Sajjad Mona Siddiqui Saima Naseem Frequency Of Bacterial Co-Infections Isolated from Covid-19 Positive Patients From Tertiary Care Hospital Of Karachi Journal of Rawalpindi Medical College COVID-19, bacterial coinfection, Pseudomonas aeruginosa, Acinetobacter baumannii, MRSA |
| title | Frequency Of Bacterial Co-Infections Isolated from Covid-19 Positive Patients From Tertiary Care Hospital Of Karachi |
| title_full | Frequency Of Bacterial Co-Infections Isolated from Covid-19 Positive Patients From Tertiary Care Hospital Of Karachi |
| title_fullStr | Frequency Of Bacterial Co-Infections Isolated from Covid-19 Positive Patients From Tertiary Care Hospital Of Karachi |
| title_full_unstemmed | Frequency Of Bacterial Co-Infections Isolated from Covid-19 Positive Patients From Tertiary Care Hospital Of Karachi |
| title_short | Frequency Of Bacterial Co-Infections Isolated from Covid-19 Positive Patients From Tertiary Care Hospital Of Karachi |
| title_sort | frequency of bacterial co infections isolated from covid 19 positive patients from tertiary care hospital of karachi |
| topic | COVID-19, bacterial coinfection, Pseudomonas aeruginosa, Acinetobacter baumannii, MRSA |
| url | https://www.journalrmc.com/index.php/JRMC/article/view/2024 |
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