Codon usage modulates the relationship between the burden and yield of protein overexpression

Abstract Background Excess utilization of translational resources is a critical source of burden on cells engineered to overexpress exogenous proteins. To improve translational efficiency, researchers often modify codon usage in an exogenous gene to more closely match the composition of a host organ...

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Bibliographic Details
Main Authors: Cameron T. Roots, Alexis M. Hill, Claus O. Wilke, Jeffrey E. Barrick
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Journal of Biological Engineering
Online Access:https://doi.org/10.1186/s13036-025-00521-z
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