Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase
Abstract Pseudouridine (Ψ) is an abundant RNA chemical modification that plays critical biological functions. Current Ψ detection methods are limited in identifying Ψs at base-resolution in U-rich sequence contexts, where Ψ occurs frequently. Here we report “Mut-Ψ-seq” that utilizes the classic N-cy...
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Nature Portfolio
2025-01-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-025-07467-4 |
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| author | Zhiyong He Weiqi Qiu Huiqing Zhou |
| author_facet | Zhiyong He Weiqi Qiu Huiqing Zhou |
| author_sort | Zhiyong He |
| collection | DOAJ |
| description | Abstract Pseudouridine (Ψ) is an abundant RNA chemical modification that plays critical biological functions. Current Ψ detection methods are limited in identifying Ψs at base-resolution in U-rich sequence contexts, where Ψ occurs frequently. Here we report “Mut-Ψ-seq” that utilizes the classic N-cyclohexyl N′-(2-morpholinoethyl)carbodiimide (CMC) agent and an evolved reverse transcriptase (“RT-1306”) for Ψ mapping at base-resolution. CMC selectively labels Ψs in RNA forming the CMC-Ψ adduct and we show that RT-1306 presents promoted read-through and mutation against the CMC-Ψ. We report a high-confidence list of Ψ sites in polyA-enriched RNAs from HEK-293T cells identified by orthogonal chemical treatments (CMC and bisulfite). The mutation signatures resolve the position of Ψ in UU-containing sequences, revealing diverse occurrence of Ψs in such sequences. This work provides methods and datasets for biological research of Ψ, and expands the toolkit for epitranscriptomic studies by combining the reverse transcriptase engineering and selective chemical labeling strategies. |
| format | Article |
| id | doaj-art-86eddebb34ed4fe59f5769283bb3cfcf |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-86eddebb34ed4fe59f5769283bb3cfcf2025-01-12T12:35:33ZengNature PortfolioCommunications Biology2399-36422025-01-018111110.1038/s42003-025-07467-4Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptaseZhiyong He0Weiqi Qiu1Huiqing Zhou2Department of Chemistry, Merkert Chemistry Center, Boston CollegeDepartment of Chemistry, Merkert Chemistry Center, Boston CollegeDepartment of Chemistry, Merkert Chemistry Center, Boston CollegeAbstract Pseudouridine (Ψ) is an abundant RNA chemical modification that plays critical biological functions. Current Ψ detection methods are limited in identifying Ψs at base-resolution in U-rich sequence contexts, where Ψ occurs frequently. Here we report “Mut-Ψ-seq” that utilizes the classic N-cyclohexyl N′-(2-morpholinoethyl)carbodiimide (CMC) agent and an evolved reverse transcriptase (“RT-1306”) for Ψ mapping at base-resolution. CMC selectively labels Ψs in RNA forming the CMC-Ψ adduct and we show that RT-1306 presents promoted read-through and mutation against the CMC-Ψ. We report a high-confidence list of Ψ sites in polyA-enriched RNAs from HEK-293T cells identified by orthogonal chemical treatments (CMC and bisulfite). The mutation signatures resolve the position of Ψ in UU-containing sequences, revealing diverse occurrence of Ψs in such sequences. This work provides methods and datasets for biological research of Ψ, and expands the toolkit for epitranscriptomic studies by combining the reverse transcriptase engineering and selective chemical labeling strategies.https://doi.org/10.1038/s42003-025-07467-4 |
| spellingShingle | Zhiyong He Weiqi Qiu Huiqing Zhou Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase Communications Biology |
| title | Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase |
| title_full | Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase |
| title_fullStr | Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase |
| title_full_unstemmed | Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase |
| title_short | Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase |
| title_sort | promoted read through and mutation against pseudouridine cmc by an evolved reverse transcriptase |
| url | https://doi.org/10.1038/s42003-025-07467-4 |
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